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蛋白酶体抑制剂联合亚砷酸对NB4细胞survivin基因表达的影响 被引量：1

Effect of proteasome inhibitor and As_2O_3 on expression of survivin mRNA in NB4 cells
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摘要 目的研究蛋白酶体抑制剂硼替佐米(bortezomib)单药应用及其联合亚砷酸(As2O3)对急性早幼粒细胞白血病(APL)细胞株NB4中survivin mRNA表达的影响。方法将受试对象分为9组:未加药空白对照组(D组);As2O3组,0.5μmol/L(A1组)和1μmol/L(A2组);bortezomib组,5nmol/L(B1组)和10nmol/L(B2组);联合组,0.5μmol/LAs2O3+5nmol/Lbortezomib(C1组),0.5μmol/LAs2O3+10nmol/Lbortezomib(C2组),1μmol/LAs2O3+5nmol/Lbortezomib(C3组),1μmol/LAs2O3+10nmol/Lbortezomib(C4组)。作用时间分为三个水平:24h,48h和72h。应用逆转录聚合酶链反应(RT-PCR)法检测药物作用前后抑凋亡基因survivinmRNA的表达水平。结果bortezomib和As2O3单药组中survivinmRNA的表达水平下降,且存在剂量与时间依赖性,联合组survivin mRNA的表达水平亦下降,且比单药组下降更明显。结论bortezomib与As2O3可通过抑制NB4细胞株survivin mRNA的表达诱导细胞发生凋亡,两药联合具有协同效应。 Objective To study the effect of bortezomib alone or combination of bortezomib and As2O3 on the expression of survivin mRNA in NB4 cells.Methods The subjects were divided into nine groups：control group（D group）;As2O3 groups：0.5 μmol/L（A1 group）and 1 μmol/L（A2 group）;bortezomib groups：5 nmol/L（B1 group）and 10 nmol/L（B2 group）;combination groups：0.5 μmol/L As2O3＋5 nmol/L bortezomib（C1 group）,0.5 μmol/L As2O3＋10 nmol/L bortezomib（C2 group）,1 μmol/L As2O3＋5 nmol/L bortezomib（C3 group）,1 μmol/L As2O3＋10 nmol/L bortezomib（C4 group）.The drug interference lasted for 24 h,48 h and 72 h,respectively.The expression of survivin mRNA was determined by reverse transcription polymerase chain reaction（RT-PCR）.Results The expression of survivin mRNA decreased in NB4 cells treated with bortezomib or As2O3 alone in a dose and time dependent manner,higher than that treated with combination of bortezomib and As2O3.Conclusion Bortezomib and As2O3 could induce apoptosis by inhibiting the expression of survivin mRNA in NB4 cells.Combination of bortezomib and As2O3 could have a synergistic effect.
作者 李亚妮 马梁明
机构地区 山西医科大学第二临床医学院血液科
出处 《山西医科大学学报》 CAS 2008年第3期218-221,共4页 Journal of Shanxi Medical University
关键词 蛋白酶体抑制剂 BORTEZOMIB AS2O3 NB4 survivin mRNA 凋亡 proteasome inhibitor bortezomib As2O3 NB4 survivin mRNA apoptosis
分类号 R733.7 [医药卫生—肿瘤]
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参考文献10

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共引文献16

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山西医科大学学报
2008年 第3期

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