人胶质瘤细胞系CHG-5和SHG-44差异表达基因的鉴定

Identification of Differentially Expressed Gene in Glioma cell lines CHG and SHG

目的:采用基因芯片技术分析不同恶性程度胶质瘤细胞CHG-5(WHO分级为级)和SHG-44(WHO分级为级)的差异表达基因。方法:抽提总RNA,进行RT-PCR,并用荧光染料Cy3和Cy5标记cDNA产物,然后进行芯片杂交,检测CHG-5和SHG-44基因表达的差异,并用Northern blot杂交来验证芯片结果。结果:与CHG-5相比,SHG-44细胞中检测到有103个基因表达明显上调,89个表达明显下调。芯片结果得到Northern blot杂交结果的支持。结论:初步揭示了胶质瘤进展的差异表达基因,为胶质瘤的进一步研究提供了基础资料。

Objective：To identify the differentially expressed genes in glioma cell lines CHG-5 （WHO grade Ⅱ ） and SHG-44 （WHO grade IV ） using cDNA microarray. Methods ：Differentially expressed genes of glioma cell lines SHG- 44 and CHG-5 were detected by cDNA microarray. Total RNA of the cell lines were extracted and reverse-transcripted. The cDNA product was labeled with Cy3＆Cy5 fluorescence dye for microarray hybridizations. NOrthern blot hybridizations were used to confirm the differentially expressed genes detected by the microarrays. Results ：Compared with the cell line CHG-5, 103 genes were up-regulated （ratio：〉2.0） and 89 genes were down-regulated （ratio〈0.5） in the SHG-44 cell line. Conclusion： The differentially expressed genes may be involved in the progression of glioma, they can provide the basic data for further research the neurogliocytoma.