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Tolerance and removal of chromium(Ⅵ) by Bacillus sp. strain YB-1 isolated from electroplating sludge

Tolerance and removal of chromium(Ⅵ) by Bacillus sp. strain YB-1 isolated from electroplating sludge
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摘要 Four chromium(Ⅵ)-resistant bacteria named YB-1,YB-2,YB-3 and YB-4 were isolated from Cr-electroplating sludge. YB-1 and YB-2 were identified as a member of Bacillus sp.based on morphology and Biolog Microstation System.The strain of YB-1 was selected to test for its resistance and ability to remove Cr(Ⅵ)from aqueous solution.The results indicate that YB-1 exhibits high MIC value which can almost reach 140 mg/L and the growth of YB-1 in liquid medium containing 60 mg/L Cr(Ⅵ)is affected especially in the late exponential phase and stationary phase.Furthermore,the potential of living and freeze-dried YB-1 biomass to remove Cr(Ⅵ)was studied in different pH,biosorbent dose,contact time and initial concentration using the batch method. At the optimal conditions,living and freeze-dried biomass are capable of absorbing 34.5 mg/g and 17.8 mg/g chromium(Ⅵ)at initial concentration of 60 mg/L,respectively.The adsorption data were fitted to Langmuir isotherm model for these two sorbents.Kinetic studies show that the rates of sorption all follow the pseudo-second order kinetics. Four chromium(Ⅵ)-resistant bacteria named YB-1, YB-2, YB-3 and YB-4 were isolated from Cr-electroplating sludge. YB-1 and YB-2 were identified as a member of Bacillus sp. based on morphology and Biolog Microstation System. The strain of YB-1 was selected to test for its resistance and ability to remove Cr(Ⅵ) from aqueous solution. The results indicate that YB-1 exhibits high MIC value which can almost reach 140 mg/L and the growth of YB-1 in liquid medium containing 60 mg/L Cr(Ⅵ) is affected especially in the late exponential phase and stationary phase. Furthermore, the potential of living and freeze-dried YB-1 biomass to remove Cr(Ⅵ) was studied in different pH, biosorbent dose, contact time and initial concentration using the batch method. At the optimal conditions, living and freeze-dried biomass are capable of absorbing 34.5 mg/g and 17.8 mg/g chromium(Ⅵ) at initial concentration of 60 mg/L, respectively. The adsorption data were fitted to Langmuir isotherm model for these two sorbents. Kinetic studies show that the rates of sorption all follow the pseudo-second order kinetics.
出处 《中国有色金属学会会刊:英文版》 CSCD 2008年第2期480-487,共8页 Transactions of Nonferrous Metals Society of China
基金 Project(04JJ3013)supported by the Natural Science Foundation of Hunan Province,China Project(20050532009)supported by theDoctoral Fund of Ministry of Education of China
关键词 张力 电镀技术 合金 Bacillus sp. Cr(Ⅵ) minimal inhibitory concentration biosorption kinetic model
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