氧化苦参碱对大鼠局灶性脑缺血再灌注损伤保护作用的中枢机制

Central mechanisms of protective effects of oxymatrine on injury induced by focal cerebral ischemia reperfusion in rats

目的:探讨氧化苦参碱(OMT)对大鼠局灶性脑缺血再灌注损伤的作用及其中枢机制。方法:采用结扎大脑中动脉的方法制备大鼠局灶性脑缺血再灌注模型。外周给药大鼠随机分为假手术组、脑缺血再灌注模型组、路路通组(LLT,31.25mg.kg-1)及OMT35、70和105mg.kg-1腹腔注射给药组;脑室给药大鼠随机分为假手术组、脑缺血再灌注模型组、LLT0.15mg.kg-1组及OMT0.35mg.kg-1脑室注射给药组。以神经学评分及脑梗塞面积为指标观察OMT对大鼠局灶性脑缺血再灌注性损伤的保护作用,同时检测OMT脑室注射给药大鼠血清NO含量。结果:与脑缺血再灌注模型组比较,腹腔注射OMT105mg.kg-1组神经学评分明显降低(P<0.05),OMT70和105mg.kg-1组脑梗塞面积缩小(P<0.05);与脑缺血再灌注模型组比较,OMT0.35mg.kg-1脑室注射给药组大鼠脑梗塞面积缩小(P<0.05),血清NO含量明显降低(P<0.05)。结论:脑室注射OMT对局灶性脑缺血再灌注大鼠的脑损伤具有明显的保护作用,中枢作用可能为其机制之一。

Objective To explore the effects and central mechanisms of oxymatrine （OMT） on injury induced by focal cerebral ischemia reperfusion in rats. Methods The focal cerebral ischemia reperfusion was induced by middle cerebral artery occlusion in rats. The rats injected by intraperitoneal were randomly divided into sham group, focal cerebral ischemia reperfusion model group, LLT 31.25 mg ·kg^-1 group, and OMT 35, 70 and 105 mg ·kg^-1 groups. The rats injected by cerebral ventricle were randomly divided into sham group, focal cerebral ischemia reperfusion model group, LLT 0. 15 mg ·kg^-1 group, and OMT 0.35 mg ·kg^-1 group. The neurological score and infarct areas were used to evaluate the protective effects of OMT in focal cerebral ischemia reperfusion rats. The NO content in serum was determined in focal cerebral ischemia reperfusion rats injected by cerebral ventricle. Results OMT 70 and 105 mg ·kg^-1 by intraperitoneal injection decreased neurological score （P〈0. 05）, deflated infarct areas （P〈0.05） compared with focal cerebral ischemia reperfusion model group. Compared with focal cerebral ischemia reperfusion model group, the infarct area and the NO content in serum were decreased in rats treated with OMT 0.35 mg ·kg^-1 by cerebral ventricle injection. Conclusion OMT injected by cerebral ventricle has protection against injury induced by cerebral ischemia reperfusion in rats. The central mechanism may be one of mechanisms of OMT .