大鼠炎症性肠病模型的复制及中药的干预作用

Reproduction of rat inflammatory bowel disease model and intervention effect of Changkangyin on it

目的:应用2,4,6-三硝基苯磺酸(TNBS)复制炎症性肠病动物模型,观察中药肠康饮(CKY)对炎症性肠病的干预作用。方法:60只大鼠随机分为乙醇对照组、模型组、柳氮磺吡啶(SASP)组及CKY低、中、高剂量组,应用TNBS复制炎症性肠病动物模型,药物干预21d后,观察大体形态学及组织病理学改变。结果:模型组体重明显低于乙醇对照组(P<0.01),CKY低、中、高剂量组及SASP组体重均高于模型组(P<0.01),CKY高剂量组体重高于SASP组(P<0.01)。模型组大体形态学及组织病理学评分明显高于乙醇对照组(P<0.01),CKY低、中、高剂量组及SASP组大体形态学及组织病理学评分均较模型组明显降低(P<0.01或P<0.05),CKY高剂量组与SASP组差异无显著性(P>0.05)。结论:TNBS可诱发出大鼠结肠炎,类似人类炎症性肠病改变,是进行炎症性肠病发病机制研究和药物疗效观察的理想模型,中药CKY对炎症性肠病大鼠模型具有较好的治疗作用。

Objective To reproduce the inflammatory bowel disease （IBD） models induced by 2, 4, 6- trinitrobenzene sulfonic acid （TNBS）, and to investigate the effect of Changkangyin （CKY） on it. Methods Total 60 rats were divided into 6 groups by random： alcohol control group, model group, salicylazosulfapyridine （SASP） group, low, middle and high dosages CKY groups. IBD model was induced by TNBS. The changes of macroscopic morphous and pathohistology of colon were observed after intervened with drug for 21 d. Results The body weight in model group was obviously lower than that in alcohol control group （P〈0.01） ; the body weights in low, middle and high dosages CKY groups and SASP group were higher than that in model group （P〈0.01） ; the body weight in high dosage CKY group was higher than that in SASP group （P〈0.01） . The scores of macroscopic morphous and pathohistology in model group were higher than those in alcohol control group obviously （P〈0.01）; the scores in low, middle and high dosage CKY groups and SASP group were lower than those in model group obviously （P〈20. 01 or P〈0.05） ; there was no significant difference between high dosage CKY group and SASP group （P〈0.05） . Conclusion Colitis can be induced by TNBS in rats. The change of it is the same as human＇s. It is a ideal model to study pathogenesis of IBD and investigate the effect of medicine. CKY has a batter therapeutic effect on IBD model.