紧密连接蛋白claudin-6在人乳腺癌细胞和组织中的表达及其与乳腺癌淋巴结转移的关系

Expression of tight junctions protein claudin-6 in breast cancer tissues and cell lines and its relationship with metastasis of breast cancer

目的:探讨紧密连接蛋白claudin-6在人乳腺癌细胞株、乳腺癌组织中的表达及与淋巴结转移的关系,为判定乳腺癌淋巴结转移及估计预后提供依据。方法:应用RT-PCR和免疫组织化学S-P法,对人乳腺上皮细胞株HBL-100、低侵袭性乳腺癌细胞株MCF-7、高侵袭性乳腺癌细胞株MDA-MB231和38例人乳腺癌组织claudin-6的表达进行检测,同时以30例乳腺良性肿瘤作为对照。结果:claudin-6在HBL-100细胞株中有表达,在乳腺癌细胞株MCF-7和MDA-MB231中均无表达;乳腺癌组织中claudin-6表达明显低于对照组(P<0.05);claudin-6表达与乳腺癌组织的病理分级及淋巴结转移有关联(P<0.05),与患者年龄和临床分期无关(P>0.05)。结论:claudin-6可能在乳腺癌的发生和转移中起一定的负性调控作用,并可作为判断乳腺癌淋巴结转移和估计预后的参考指标。

Objective To investigate the expression of tight junctions protein claudin-6 in breast cancer cell lines and tissues and its relationship with metastasis. Methods RT-PCR and immunohistochemistry analysis were used to detect claudin-6 mRNA and protein expressions in human mammary cancer tissues and cell lines. Results claudin-6 gene was expressed in mammary gland epithelial cell HBL-100, but undetectable in other cell lines; compared with benign mammary gland, fibroadenoma, claudin-6 was down-regulated in breast cancer （P〈0.05）. Furthermore, the down-regulation had significantly negative correlation with mammary tumor pathological grades and metastasis （P〈0. 05）, but not related to agc and clinical stages （P〉0.05）. Conclusion claudin-6 may play a negative regulatory effect on mammary carcinogenesis and metastasis, and may be a potential indicator for mammary cancer metastasis and prognosis.