2型17β羟化类固醇脱氢酶在乳腺癌和癌旁组织中的表达及其与临床特征的关系

Expression of 17β-hydroxysteroid dehydrogenase type 2 in breast cancer and adjacent non-malignant tissue:a correlation to clinical factors

目的:探讨2型17β羟化类固醇脱氢酶(2型17β-HSD)在乳腺癌和癌旁非恶性乳腺组织中的表达及其与患者临床特征之间的关系。方法:采用免疫组织化学方法检测2型17β-HSD在76例(全部为女性)乳腺癌及癌旁非恶性乳腺组织中的表达,分析2型17β-HSD表达与患者年龄、癌组织雌激素受体(ER)、孕激素受体(PR)、P185、肿瘤分期、肿物大小以及阳性淋巴结数之间的关系。结果:2型17β-HSD在乳腺癌细胞的细胞浆中有表达,阳性率为5.3%;在癌旁非恶性乳腺组织腺泡上皮细胞以及导管上皮细胞的细胞浆中表达,阳性率为82.9%。乳腺癌细胞表达阳性率与癌旁乳腺组织中上皮细胞表达的阳性率比较差异有显著性(χ2=92.908,P<0.001)。2型17β-HSD在癌旁乳腺组织中的表达与乳腺癌原发肿物的大小(r=-0.341,P<0.05)和肿瘤的分期呈负相关(r=-0.706,P<0.01),与患者年龄呈正相关(r=0.677,P<0.01),而与ER、PR、P185和阳性淋巴结数之间均无相关性。在乳腺癌组织中该酶与患者年龄、ER、PR、P185、肿瘤分期、肿物大小以及阳性淋巴结数量之间均无相关性。结论:2型17β-HSD可调节乳腺局部雌激素水平,与乳腺癌发生及发展有关。

Objective To study the expression of 17β-hydroxysteroid dehydrogenase （17β-HSD） type 2 in breast cancer and adjacent non-malignant tissue, and the correlations between the results and clinical factors. Methods The expressions of the oxidative enzyme 17β-HSD type 2 in 76 specimens of female human breast cancer and adjacent non-malignant tissues were determined by immunohistochemistry. The relationships between the clinical parameters including patient＇s age, ER, PR, P185, the stage of breast cancer, tumor size and the percentage of involved lymph nodes were analyzed. Results 17β-HSD type 2 was found in the cytoplasm of tumor cells in 5.3%of cases. In adjacent non-malignant tissues, 82.9% of cases were positive and the enzyme was detected in the cytoplasm of epithelial ceils of the acini and ducts. There was a significant difference between the malignant tissue and the adjacent non-malignant tissue in the percentage of positive cells （χ2=92. 908, P〈20. 001）. In adjacent non-malignant tissues, the expression of 17β-HSD type 2 was negatively correlated with the tumor size （r=-0.341, P〈0.05）, the stage of the Breast cancer （r=-0. 706, P〈0.01） and was positively correlated with the patient＇s age （r=0. 677, P〈0.01） . For the other clinical parameters including ER, PR, P185 and the percentage of involved lymph nodes, there was no significant correlation. No significant correlation was found Between the expression of 17β-HSD type 2 and all clinical parameters in Breast cancer tissue. Conclusion 17β-HSD type 2 can regulate the local estrogen level. It may play a significant role in the development and/or progression of Breast cancer.