摘要
目的探讨肿瘤坏死因子α(TNF-α)对稳定表达人脂联素3T3-L1细胞过氧化物酶体增殖物激活受体(PPAR)γ2mRNA表达的影响,为进一步研究脂联素功能提供了实验基础。方法重组脂联素真核表达质粒(pcDNA3·1+-hADPN)脂质体法稳定转染3T3-L1细胞。用TNF-α(100ng/ml)处理未转染、转染空载载体、转染pcDNA3·1+-hADPN的3T3-L1细胞,RT-PCR检测PPARγ2mRNA的表达量。结果(1)稳定转染了pcDNA3·1+-hADPN的未分化和已分化3T3-L1细胞中,PPARγ2表达量较未转染组明显增加(P<0·01)。(2)TNF-α可明显抑制PPARγ2mRNA的表达(P<0·05)。(3)稳定转染pcDNA3·1+-hADPN可改善TNF-α抑制作用(P<0·05)。结论稳定转染pcDNA3·1+-hADPN可明显增加未分化和已分化的3T3-L1细胞PPARγ2mRNA表达。TNF-α抑制PPAR-γ2mRNA表达,而转染pcDNA3·1+-hADPN可改善TNF-α抑制作用。
Objective To observe effect of TNF-α on PPARγ2 mRNA expression in 3T3-L1 cells transfected with human recombinant adiponectin. Methods The 3T3-L1 preadipocytes were transfected with the recombinant plasmid pcDNA3. 1 ^＋- hADPN. The PPAR72 mRNA expression was quantitated by semi-quantitative RT-PCR. Results （1）Compared with controls（the 3T3-L1 cells and the 3T3-L1 cells with plasmid）, the PPAR-γ2 mRNA expression of 3T3-L1 cells with human recombinant adiponectin was higher（P〈0. 01）. （2） In 3T3-L1 cells,3T3-L1 cells with plasmids,and 3T3-L1 cells with human recombinant adiponectin,the treatment with 100ng/ml of TNF-α decreased PPARγ2 mRNA expression compared with untreated controls（P〈0. 05）. （3） The effect of TNF-α on PPARγ2 mRNA expression in 3T3-L1 cells was reversed by stably transfected human recombinant adiponectin （P〈0. 05）. Conclusions The 3T3-L1 cells stably transfected with human recombinant adiponectin increase PPARγ2 mRNA expression in the 3T3-L1 cells as compared with controls. TNF-α suppresses PPARγ2 mRNA expression in the 3T3- L1 cells. The effect of TNF-α on PPARγ2 mRNA expression in 3T3-L1 cells is reversed by stably transfected human recombinant adiponectin.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2008年第3期156-158,共3页
Chinese Journal of Diabetes
基金
安徽省自然科学基金资助项目(03043719)
