丁酸钠对大鼠小肠上皮细胞株-6细胞增殖和凋亡的影响

Effects of sodium butyrate on the proliferation and apoptosis of intestinal epithelial cell line No. 6 of rats

目的观察丁酸钠对大鼠小肠上皮细胞株～6（intestinal epithelium cell line No．6，IEC-6）细胞增殖和凋亡的作用。方法不同浓度丁酸钠（低浓度2mmol／L，中浓度8mmol／L，高浓度14、20mmol／L）处理IEC-6细胞，细胞计数试剂盒CCK-8法检测细胞增殖情况，分光光度法检测细胞内Caspase-3活性程度，流式细胞术检测细胞凋亡率。结果（1）加入丁酸钠（2、8、14、20mmol／L）共培养24h后，2、8mmol／L丁酸钠组细胞增殖速度比对照组快，差异有统计学意义（P〈0．01）；48h后，14、20mmol／L丁酸钠组细胞增殖较对照组明显受抑制，差异有统计学意义（P〈0．01）；72h后，8、14、20mmol／L丁酸钠组增殖亦明显受抑制，与对照组比较差异有统计学意义（P〈0．01）。（2）加入丁酸钠（2、8、14mmol／L）共培养12h后，8、14mmol／L丁酸钠组与对照组比较Caspase-3活性明显升高，差异有统计学意义（P〈0.05）；24h后，2、8、14mmol／L丁酸钠组与对照组比较Caspase-3活性均升高，差异有统计学意义（P〈0.05）；48h后，8、14mmol／L丁酸钠组与对照组比较Caspase-3活性亦升高，差异有统计学意义（P〈0.05）。（3）加入丁酸钠（2、8、14mmol／L）共培养12、24、48h后，8、14mmol／L丁酸钠组与对照组比较IEC-6细胞凋亡率均有升高，差异有统计学意义（P〈0.05）。结论中、高浓度丁酸钠有诱导IEC-6细胞凋亡，抑制IEC-6细胞增殖作用，而低浓度丁酸钠没有这种作用。

Objective To investigate the effects of sodium butyrate on the proliferation and apoptosis of intestinal epithelium cell line No. 6 （IEC-6）of the rats. Methods IEC-6 cells were treated with sodium butyrate at different concentrations （low concentration 2 mmol/L, medium 8 mmol/L, high 14 and 20 mmol/L） or incubated in medium alone as a control. Morphological changes of IEC-6 cells were observed by inverted microscope, and cell proliferation was assessed by cell counting kit-8 （CCK-8） assay. Caspase-3 activity was measured and flow cytometry was used to assay the apoptosis. Results （1） The growth rates of the ceils treated with sodium butyrate in concentrations of 2, 8 mmol/L for 24 h were higher than those of control group （P〈0. 01） . At 48 h, the 14, 20 mmol/L sodium butyrate groups had the lower growth rates than control group （P〈 0.01）. At 72 h, the 8,14, 20 mmol/L sodium butyrate groups had the lower growth rates than control group （P〈0.01）. （2） The caspase-3 activity from cell lysates of IEC-6 cells treated with sodium butyrate in concentrations of 8, 14 mmol/L for 12 h were higher than that of control group （P 〈0.05） . At 24 h, the 2, 8,14 mmol/L sodium butyrate groups had the higher caspase-3 activity than control group （P〈0.05） ; At 48 h, the 8, 14 mmol/L sodium butyrate groups had the higher caspase- 3 activity than control group （P〈0. 05）. （3） The rates of cell apoptosis in groups treated with sodium butyrate in concentrations of 8, 14 mmol/L for 12, 24, 48 h were higher than those of control group （P〈0.05）. Conclusions Sodium butyrate induces apoptosis and inhibits the proliferation in rat intestinal epithelial cells, and the effects are dose and time dependent.