萃取/薄层色谱分离/闪烁记数法分析丁基锡化合物

Analysis of Butyltin Compounds by Extraction/TLC Separation/Scinticounting.

为了应用同位素示踪技术研究三丁基锡化合物在环境中的迁移转化规律，建立了萃取／薄层色谱分离／闪烁记数法分析１４Ｃ标记丁基锡的方法．研究了薄层色谱分离条件以及影响因素，首次测定了１４Ｃ标记三丁基锡在水、底泥、生物等环境样品中的回收率．结果表明，随着展开剂中极性组分乙酸的比例加大，丁基锡化合物的Ｒｆ值升高；当异丙醚、乙酸的比例为９７１时，分离效果最佳，温度对Ｒｆ值影响不明显；二丁基锡和一丁基锡的Ｒｆ值受萃取螯合剂酚酮的影响；１４Ｃ标记三丁基锡在水、水过０．４５μｍ滤膜、底泥和生物样品中的回收率分别为：４４．２％，３９．６％，３７．９％和４０．５％．

In order to study the transport and transformation of tributyltin in the aquatic environment with 14 C carbon isotope tracer technique,author developed an analytical method of Extraction/TLC Separation/Scinticounting for the determination of 14 C labelled butyltin compounds.TLC separation conditions and influence factors were investigated.The recoveries of 14 C labelled butyltin compounds in the environmental samples such as water,sediment and biota were determined for the first time.The experimental result showed that the R f value of butyltin compounds increases with the increasing proportion of polar component(acetic acid)in the developer.When the ratio isopropyl ether to acetic acid is 97∶1,optimum separation is achieved.The influence of temperature on R f value is not significant.The R f value of di and monobutyltin is influenced by the extracting complex agent,tropolone.The recoveries of 14 C labeled tributyltin in water,water filtered with a 0 45μm filter membrane,sediment and biota sample are 44 2%,39 6%,37 9%,and 40 5% respectively.