正交设计法优化从车前草中萃取熊果酸的工艺研究

Study on Technology of Extracting Ursolic Acid in Herba Plantagin by Orthogonal Designing Method

用正交实验法优化采用超临界CO2技术萃取车前草中熊果酸的工艺条件。在单因素实验基础上,采取L9(33)探讨萃取压力、萃取温度、CO2流量对萃取车前草熊果酸的得率的影响情况,确定了超临界CO2萃取车前草中熊果酸的适宜条件。超临界萃取最优条件为:萃取压力35MPa,萃取温度为40℃,选择无水乙醇为夹带剂,夹带剂质量分数为20%,萃取时间2h,CO2流量为20L/h,产品的出膏率为3.58%,萃取物中熊果酸的含量为6.93%。该法与传统方法相比,具有操作简便快速,溶剂用量少,有效成分提取率高等优点。

An orthogonal design L9（3^3） was used with the factor of extracting pressure, temperature, CO2 flow rate types. The optimum conditions for SFE CO2 were determined. The optimum extraction conditions were as follows：pressure 35.0MPa, temperature 40 ℃, modifier alcohol, at dosafe of 20 % of raw material, time 2h and flow rate of CO2 20 L/h. the yield of product was 3.58 %, the yield of Ursolic Acid in product was 6.93 %. The method had the advantages of simple operation, less solvent, Consumption, short extraction time, high extraction yield.