系统性硬皮病患者血清免疫球蛋白和补体检测及其意义

Significance of detection immunoglobulin and complement in serum of patients with systemic scleroderma

目的：测定系统性硬皮病（systemic scleroderma,SSc）患者血清免疫球蛋白IgG、IgA、IgM和补体C3、C4水平,探讨两者与SSc的关系及其在临床治疗中的意义。方法：利用速率散射比浊法,对65例初诊SSc患者治疗前后和35名健康成年人的血清IgG、IgA、IgM和补体C3、C4水平进行检测,采用t检验进行统计学分析。结果：SSc患者治疗前血清IgG、IgA水平显著升高,C3、C4水平降低,与正常人差异均有统计学意义（P〈0.01-P〈0.001）;SSc患者治疗后血清IgG、IgA和补体C3、C4水平均有变化（P〈0.05-P〈0.01）,而血清IgM水平变化不明显（P〉0.05）。结论：免疫球蛋白和补体的变化同SSc的发生发展密切相关,其血清水平的检测有助于SSc发病机制的探讨,有助于临床对SSc免疫治疗后的疗效观察。

Objective：To determine the levels of IgG, IgA, IgM and C3, C4 in serum of patients with systemic scleroderma （SSc） ,and to investigate the relations between the immune indexes and SSc, and the significance of these indexes for clinical therapy as well. Methods：The above-mentioned immune markers were examined by scatter turbidimetry in 65 preliminarily diagnosed cases of SSc before and after treatment and 35 cases of healthy controls. And all the data were examined by t-test. Results： In SSc, the pre- therapeutic IgG and IgA were increased prominently;C3 and C4 were decreased;the variance had statistical significance compared with the health controls（ P 〈 0.01 - P 〈 0.001 ）. Great differences were found in IgG, IgA, C3 and C4 in the 65 patients with SSc before and after therapy （P 〈 0. 05 - P 〈 0. 001 ） ; IgM showed no change （ P 〉 0. 05 ）. Conclusions： The change of immune globulin and complement is closely correlated to the progress of SSc. Detection of their amount is helpful to both pathogenesis exploration and effect observation.