黏着斑激酶基因沉默促进舌癌细胞失巢凋亡的实验研究

Focal adhesion kinase gene silencing promotes anoikis in human tongue cancer cell

目的观察黏着斑激酶(focal adhension kinase,FAK)基因沉默对口腔舌癌细胞株Tca8113失巢凋亡抗性的影响,探讨FAK基因与口腔鳞癌转移特性的关系。方法以舌癌细胞株Tca8113为研究对象,首先应用免疫细胞化学和免疫蛋白印记的方法检测FAK基因的表达,然后利用RNA干扰的方法抑制FAK基因的表达。最后利用悬浮培养、流式细胞仪的方法观察FAK对口腔舌癌细胞株Tca8113体外培养过程抗失巢凋亡的影响。结果舌癌细胞株Tca8113体外培养过程中具有较强的抗失巢凋亡特性。特异性siRNA可以抑制FAK基因的表达。FAK基因干扰后,Tca8113细胞的失巢凋亡显著提高(P<0.01)。结论FAK基因沉默可以逆转舌癌细胞株Tca8113体外培养过程中的抗失巢凋亡特性。

Objective To test whether FAK gene silencing can promote anoikis and reverse acquired anoikis resistance in human tongue cancer cells. Methods FAK expression was assessed by immuocytochemistry and Western blot analysis. Anoikis was induced in Tca8113（ tongue squamocarcinoma cell） with the use of polyHEMA culture. FAK expression was suppressed by RNA interference. Anoikis was detected by flow cytometry. Results The cell line that was tested showed marked anoikis resistance and greater resistance associated with higher levels of FAK expression. FAK gene silencing promoted anoikis remarkably in TcaS113（ P 〈 0.01 ）. Conchmon FAK gene silencing suppresses anoikis resistance in tongue cancer cell. FAK represents a potential target for novel antimetastatic therapies.