摘要
目的探讨应用SELDI质谱技术对胰腺癌患者血清中特异性标志蛋白的筛选方法。方法采用表面增强激光解析/离子化飞行时间质谱(SELDI-TOF-MS)技术,选择WCX磁珠及蛋白芯片阅读机对胰腺癌及健康人(对照组)和胰腺良性疾病组的血清进行检测,以筛选胰腺癌患者血清中特异表达差异蛋白。结果发现胰腺癌血清表达差异的潜在标志物(蛋白)4个,相对分子质量分别为5705Da,4935Da,5318Da和3243Da,其中4935Da,3243Da差异表达蛋白质在对照组中低表达,而在胰腺癌组中高表达,5705Da和5318Da差异表达蛋白质在胰腺癌组中低表达而对照组中高表达。结论应用SELDI技术筛选胰腺癌患者血清中的特异性生物标记物的方法快速、有效;检测到的4个差异蛋白质可能是胰腺癌患者血清特异性生物标记物。
Objective To explore the methode to screen for relatively specific markers in serum of pancreatic cancer patients using surface-enhanced laser desorption and ionization time of fight mass spectrometry （ SELDI- TOF-MS）. Methods Serum samples from 20 pancreatic cancer patiants, 20 healthy volunteers and 18 patients with other pancreatic diseases were examined. WCX magnetic beans and PBS Ⅱ-C protein chips reader （ciphergen Biosystems Ins. ） were used to detect the protein fingerprint expression of all the serum samples, and the resulting profiles were analyzed with Biomarker Wizard system. Results Four differently expressed potential biomarkers were identified with the relative molecular weights of 5 705Da, 4 935Da, 5 318Da and 3 243Da respectively. Compared with control group, two proteins with m/z 5 705Da and 5 318Da were down-regulated, and two proteins with m/z 4 935Da and 3 243Da were up-regulated in pancreatic cancers. Conclusions SELDI technology is a quick, easy and practical method to screen for specific biomarkers in serum of patients with pancreatic cancer. Four different proteins identified in this study may be as specific serum biomarkers of pancreatic cancer.
出处
《中国普通外科杂志》
CAS
CSCD
2008年第3期242-245,共4页
China Journal of General Surgery
基金
辽宁省科技攻关课题(2005225003-3)
