类肝素酶在胰腺癌组织中的表达意义

Significance of heparanasel expression in human pancreatic cancer

目的探讨胰腺癌组织中类肝素酶（heparanase 1，Hpa1）表达与胰腺癌侵袭性、临床病理等因素之间的关系。方法采用逆转录-聚合酶链反应（RT-PCR）、免疫组化（SP）法检测37例胰腺癌患者癌组织、癌旁组织及正常胰腺组织中Hpa1表达，并统计分析Hpa1表达与胰腺癌侵袭性、临床病理等因素之间的相关性。结果Hpa1在胰腺癌组织、癌旁组织、正常胰腺组织中的表达阳性率分别为64．9％（24／37）、56．8％（21／37）和10．8％（4／37）。胰腺癌组织中Hpa1表达与胰腺癌TNM分期、侵及神经和血管情况、包膜完整情况以及淋巴转移明显相关（P〈0．05），但与患者年龄、性别、组织学分化程度、肿瘤大小无关（P〉0．05）。结论Hpa1与胰腺癌发生、发展密切相关，Hpa1表达与胰腺癌的侵袭性、局部浸润、临床分期以及区域淋巴结转移有密切关系，胰腺癌Hpa1过表达可促进胰腺癌浸润生长与转移，其表达对评价胰腺癌预后亦有一定的价值。

Objective To investigate the expression of heparanasel（Hpa1） and its association with factors of invasive ability and clinical pathological features. Methods The expression of Hpa1 in cancerous, paracancerous and normal tissues from 37 patients with pancreatic cancer were examined by RT-PCR and immunohistochemistry. The correlation of Hpa1 expression with invasive ability and clinico-pathological features was analyzed. Results The positive rates of Hpa1 expression in cancerous, paracancerous and normal tissues were 64.9% （24/37）, 56.8% （21/37）, 10.8% （4/37）, respectively. The expression of Hpa1 was correlated with TNM grade, invasion of nerves or vascellum, peplos integrity and lymphoid metastasis （P 〈 0.05）, but unrelated with age, gende, histological differentiation and tumor size （P 〉0. 05）. Conclusions The Hpa1 involved in the oncogenesis and development of pancreatic cancer. Hpa1 expression is significant associated with invasive ability, local infiltration, clinical stage and lymphoid metastasis.