血小板反应素-1的表达与胰腺癌转移及预后

Relationship of TSP-1 expression with metastasis and prognosis of human pancreatic cancer

目的:探讨血小板反应素-1(TSP-1)在胰腺癌转移及预后评价中的价值。方法:应用免疫组化方法检测74例胰腺癌组织和16例其他胰腺组织(6例正常胰腺组织、4例慢性胰腺炎组织、6例癌旁正常胰腺组织)中TSP-1的表达与分布,所得数据用SPSS 11.0软件进行统计学处理,分析胰腺癌组织中TSP-1表达对判断胰腺癌转移及预后的价值。结果:TSP-1在胰腺癌组织中的表达明显高于正常胰腺及慢性胰腺炎组织(P<0.05)。不同肿瘤分期、不同肿瘤大小、有无淋巴结转移、有无神经浸润胰腺癌患者TSP-1表达有统计学差异(P<0.05);Logistic逐步回归分析显示,TSP-1表达与肿瘤分期、神经浸润密切相关。Kaplan-Meier方法和Log-rank检验分析显示,TSP-1表达、淋巴结转移、神经浸润、肿瘤分期与肿瘤术后生存率相关;多变量COX风险模型分析显示,神经浸润、淋巴结转移与肿瘤生存预后直接相关,而TSP-1表达不是直接相关因素。结论:TSP-1可以作为判断胰腺癌是否转移的指标,对判断胰腺癌预后有一定临床价值。

Objective：To study the relationship of TSP-1 expression with the metastasis and prognosis of human pancreatic cancer. Methods： The expression and distribution of TSP-1 were investigated in 74 pancreatic cancer tissues and 16 other pancreatic tissues （6 normal pancreatic tissues, 4 chronic pancreatitis tissues and 6 normal pancreatic tissues adjacent to the pancreatic cancer tissues） using immunohistochemical method. The data were analyzed by using SPSS 11.0 software and the value of TSP-1 expression in predicting the metastasis and prognosis of pancreatic cancer was investigated. Results： The expression of TSP-1 in the pancreatic cancer tissues was significantly higher than that in the normal pancreatic and chronic pancreatitis tissues （P〈0.05）. Significant differences were also found between tumors of different stages, different sizes, with or without lymphatic metastasis, and with or without neural infiltration （P〈0.01 or P〈0.05）. Logistic regression analysis showed that TSP- 1 expression was correlated with the stages and neural infiltration of the tumor. Univariate analysis using the Kaplan-Meier method and log-rank test revealed a relationship of TSP-1 expression with lymphatic metastasis, neural infiltration, tumor stage and patient survival rate. Multivariate analysis using the Cox proportional hazard model demonstrated that the neural filtration, lymphatic metastasis were the independent factors of patients＇ prognosis and survival; TSP-1 expression was not independent prognostic parameter. Conclusion： Expression and distribution of TSP-1 may serve as indicator for metastasis of pancreatic cancer and is also helpful in predicting the prognosis of pancreatic cancer.