沙利度胺对人肝癌细胞株PPARγ、COX-2表达的影响

EFFECTS OF THALIDOMIDE ON PPARγ AND COX-2 EXPRESSION OF HUMAN HEPATOMA CELL LINES

目的观察沙利度胺对人肝癌细胞系HepG2细胞过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor-γ,PPARγ)、环氧合酶-2(cyclooxygenase-2,COX-2)表达的影响。方法应用免疫细胞化学法观察沙利度胺50、100、200、400 mg/L分别作用12、24、48、72 h对HepG2细胞PPARγ、COX-2表达的影响。结果沙利度胺作用于HepG2细胞可上调PPARγ表达,激活PPAR通路并降低COX-2表达,以200 mg/L作用48h效果最为明显。结论沙利度胺对人肝癌细胞株PPARγ、COX-2表达有明显作用,可能是沙利度胺抗肿瘤机制之一。

Objective To investigate the effects of thalidomide at different concentrations on peroxisome proliferator-activated receptor-γ （ PPARy ） and cyclooxygenase-2 （ COX-2 ） expression in human hepatoma cell lines HepG2 in vitro . Methods Thalidomide was given in vitro to HepG2 which were randomly divided into 5 groups, untreated control group; thalidomide group （50,100,200,400 mg/L） ; After HepG2 treated for 12,24,48,72 hours as described above, the effects of treatments were studied by modulation of gene expression of PPARγ, COX-2 immunohistochemistry. Results The results of immunohistochemical technique showed that thalidomide could up-regulate the expression of PPARγ and down-regulate the expression of COX-2 in vitro . Thalidomide 200 mg/L group showed the best treating effect on HepG2 cell lines after treating 48 hours. Conclusion Thalidomide can obviously increase PPARγ expression, deregulate COX-2 gene expression in HepG2 cell lines in vitro ,which maybe one of the possible molecular antitumor mechanisms of thalidomide.