摘要
目的:建立高效液相色谱法测定丹芪降糖胶囊中丹参酮ⅡA含量的方法。方法:采用Symmetry C18色谱柱(4.6mm×250mm),以甲醇-水(80:20)为流动相,流速为1mL/min,检测波长270nm。结果:丹参酮ⅡA的线性范围为0.064mg~0.320mg,(r=0.9998),平均回收率为99.4%(RSD=2.58%)。结论:本方法简便、准确,可作为丹芪降糖胶囊的质量控制依据。
Objective:To establish a method for determining the content of tanshinone Ⅱ A in Danqi Jiangtang capsule by HPLC.Method:HPLC was performed on Symmetry C18(4.6 mm × 250 mm)column with methanol-water(80 : 20) as mobile phase at flow rate of 1 mL/min.The detection wavelength was 270 nm.Results:The liner range of tanshinone 11 A was 0.064 mg- 0.320 mg (r= 0.999 8 ), the average recovery rate was 99.4% and RSD was 2.58%.Conclusion:The method is simple, sensitive and can be used for the analysis of tanshinone ⅡA in Danqi Jiangtang capsule.
出处
《山西中医学院学报》
2008年第1期47-48,共2页
Journal of Shanxi College of Traditional Chinese Medicine