高效液相色谱法测定丹芪降糖胶囊中丹参酮ⅡA的含量

Determination of TanshinoneⅡA in Danqi Jiangtang Capsule by HPLC

目的:建立高效液相色谱法测定丹芪降糖胶囊中丹参酮ⅡA含量的方法。方法:采用Symmetry C18色谱柱(4.6mm×250mm),以甲醇-水(80:20)为流动相,流速为1mL/min,检测波长270nm。结果:丹参酮ⅡA的线性范围为0.064mg～0.320mg,(r=0.9998),平均回收率为99.4%(RSD=2.58%)。结论:本方法简便、准确,可作为丹芪降糖胶囊的质量控制依据。

Objective：To establish a method for determining the content of tanshinone Ⅱ A in Danqi Jiangtang capsule by HPLC.Method：HPLC was performed on Symmetry C18（4.6 mm × 250 mm）column with methanol-water（80 ： 20） as mobile phase at flow rate of 1 mL/min.The detection wavelength was 270 nm.Results：The liner range of tanshinone 11 A was 0.064 mg- 0.320 mg （r= 0.999 8 ）, the average recovery rate was 99.4% and RSD was 2.58%.Conclusion：The method is simple, sensitive and can be used for the analysis of tanshinone ⅡA in Danqi Jiangtang capsule.