辽宁各地酸浆宿存萼中木樨草素含量测定

Determination of Luteolin in Physalis Alkekengi Sepal by HPLC

目的:对辽宁省20个产点的酸浆宿存萼中木樨草素的含量进行比较。方法:采用高效液相色谱法进行木樨草素含量测定。结果:木樨草素在(0.0286～0.1430)μg成良好的线性关系(r=0.9995),加样回收率为99.04%,RSD=2.83%(n=5)。结论:相同成熟度,相同的生态环境,但不同地点的酸浆宿存萼中木樨草素含量存在差异。

Objective：To determine luteolin content in Physalis alkekengi sepal which were collected from 20 places in Liaoning province. Method：HPLC was performed on Eclipse XDB-C18（4.6 mm × 150 mm）column with methanol-water （1：1） as mobile phase at flow rate of 1.0 mL/min. The column was 25 ℃ and the detection wavelength was 349 nm. Results：The liner range of luteolin was 0.028 6 mg-0.143 0 mg （r=0.999 5）,the average recovery rate was 99.04% and RSD was 2.83%（n=5 ）.Conclusion：There exists significant difference in the content of luteolin in Physalis alkekengi sepal which was collected from different places.