丁香酚对灰葡萄孢的抑制作用研究

Inhibition Activity of Eugenol to Botrytis cinerea

丁香酚是从植物中提取的天然化合物。采用茵丝生长速率法测定了其对多种植物病原真菌的抑制作用以及田间分离得到的7株灰葡萄孢Botrytiscinerea对丁香酚的敏感性，同时测定了丁香酚对B．cinerea03孢子和菌核的抑制活性。结果显示，丁香酚对多种植物病原真菌均有不同程度的抑制作用；灰葡萄孢不同菌株对丁香酚的敏感性不同，EC50值在29．97-83．62wg／mL之间；丁香酚可抑制灰葡萄孢产孢，但对孢子萌发无影响，可抑制其菌核的产生和萌发。荧光染色结果表明丁香酚能破坏灰葡萄孢菌丝细胞膜。利用原子吸收光谱法和紫外分光光度法分别测定丁香酚处理前后菌丝培养液中K^＋浓度和OD260值变化，结果显示处理后K^＋浓度和OD260值均升高。综上所述，细胞膜可能是丁香酚对灰葡萄孢菌丝的作用位点。

The antifungal activity of eugenol （a natural compound） against 10 fungal pathogens including Botrytis cinerea was detemined by mycelial growth rate method. The results showed the sensitivity of 7 strains of B. cinerea to eugenol was different, and the ECs0 values were ranging from 29.97 to 83.62 μg/mL. Eugenol could reduce the sclerotium number, germination and sporulation, but had no effect on spores germination of B. cinerea. Hyphae of B. cinerea treated with eugenol showed strong propidium iodide fluorescence in the cytosol viewed with a fluorescence microscope. Eugenol could also increase the concentration of potassium ion and cellular materials in the medium. The results suggested that the cell membrane might be the target of eugenol.