HPLC检测猪肉组织中3种氟喹诺酮类药物残留

Simultaneous determination of three fluoroquinolone residues in pork by HPLC

建立了HPLC-FLD同时检测猪肉组织中环丙沙星、单诺沙星、沙拉沙星等3种氟喹诺酮类药物。猪肉组织样品用磷酸盐水溶液提取,在C18固相萃取柱净化,采用乙腈-磷酸/三乙胺溶液作为流动相,荧光检测器检测(λex=280 nm,λem=450nm)。这3种氟喹诺酮标准曲线的线性回归系数均在0.9999以上,检测线性范围环丙沙星、沙拉沙星为(2.5～500)μg/kg,单诺沙星为(0.5～100)μg/kg。猪瘦肉组织中这3种氟喹诺酮的平均加标回收率为79.9%～93.6%;相对标准偏差分别为2.54%、2.60%、2.51%。环丙沙星、沙拉沙星的最低检测限为2.5μg/kg,单诺沙星的最低检测限为0.5μg/kg。

Three fluoroquinolone residues including ciprofloxacin, danofloxacin, sarafloxacin in pork were simultaneously determined by HPLC （High performance liquid chromatography） with fluorescence detection （FLD）. The samples were extracted with phosphate aqueous solution and purified with C18 solid phase extraction column. Then, the extractions were separated with acetonitrile-phosphorous acid/tri-ethylamine system as the mobile phase, and detected by FLD （λex=280nm, λem=450nm）. The linear ranges for ciprofloxacin and saratloxacin were （2.5-500）μg/kg, however that for danofloxacin was （0.5-100）μg/kg. All of their correlation coefficients were larger than 0.9999, and the average recoveries for those three fluoroquinolone residues in pork were from 79.9% to 93.6%. Their relative standard deviations are 2.54%, 2.60%, 2.51% respectively. And the LODs for ciprofloxacin and sarafloxacin were 2.5 μg/kg, however that for danofloxacin was 0.5 μg/kg.