橙花长寿花叶柄组织培养技术研究

Research on Tissue Culture and Plantlet Reproduction From Leafstalk of Kalanchoe blossfeldiana with Orange Flower

以橙花长寿花叶柄作为外植体,对其进行不定芽诱导及增殖培养研究,结果表明:最适宜叶柄分化的培养基为MS+6-BA0.5mg/L+NAA2.0mg/L,最适合芽增殖的培养基为MS+6-BA0.5mg/L+NAA0.4mg/L,生根培养基为1/2MS+IBA0.2mg/L,试管苗移栽基质中成活率达98%。

The inducing adventitious shoots and proliferation culture of Kalanchoe blossfeldiana was studied by using the leafstalks as explants .The results indicated that the most appropriate media in culture leafstalks was MS＋BA 0.5 mg/L ＋ NAA 2.0 mg/L; The appropriate medium for inducing buds from explants was MS＋BA 0.5 mg/L ＋ NAA0.4 mg/L and the most appropriate medium of I/2MS＋ IBA 0.2 mg/ L for rooting. The plantets are transplanted into nutrient matrix with 98% survival ratio.