荧光检测碱性磷酸酶活力方法的建立

Establishing the Testing Method of ALP Activity with Fluorometry

以4-甲基伞形酮磷酸酯为底物，利用动力学模式，建立了一种检测碱性磷酸酶活力的荧光法。确定了荧光检测的最佳条件为：激发波长为365nm，发射波长为449nm，作用时间为第2～6min，pH值为9，底物体积为100μL，底物缓冲溶液浓度为0．1mol·L^-1。该法操作简便、快速。

Taking 4-methylumbelliferyl phosphate liquid （4-MUP） as substrate, the fluorometric method of alkaline phosphatase （ALP）activity has been established with the kinetics pattern. The optimum detection conditions are determined as follows： the excitation wavelength is 365 nm and emission wavelength is 449 nm;the reaction time is 2th-6th min;the pH value is 9; substrate volume is 100μL, concentration of substrate buffer solution is 0. 1 mol · L^-1. The method is simple and fast.