兔VX-2肝移植肿瘤的制作方法研究

Study of preparation of rabbit model with VX-2 carcinoma in liver

目的探索应用不同方法制作VX-2兔移植性肝癌模型,评价原位成瘤率。方法新西兰白兔38只,采用VX-2瘤株动物自身接种传代。A组15只采用超声引导瘤组织混悬液注射法;B组13只采用开腹瘤组织混悬液注射法;C组10只采用瘤组织块包埋法,均接种于肝左叶。各组分别于接种后1周、2周、3周、4周、5周行CT平扫、增强扫描及MR I平扫。计算各组的原位成瘤率。结果A、B组各种植成功10只,C组种植成功7只,分别为66.7%、76.9%、70%,无统计学意义。结论三组制作方法都有较高的原位成瘤率,实验周期短,均可用于兔VX-2肝癌模型的制作。

Objective： To study the methods of preparing the rabbit model with VX-2 carcinoma in liver used in the experimental radiology and evaluate the original growth rate of each group. Methods： 38 New-Zealand white rabbits were divided into 3 groups. 15 New-Zealand white rabbits were inoculated with suspension with Ultrasound transduce, 13 were inoculated with suspension by laparotomy and 10 were inoculated with VX-2 carcinoma in the liver by laparotomy implantation. All were inoculated in the left lobe of the liver and examined by plain and dual-phase enhanced CT scan and MRI plain scan respectively in 1 week, 2 weeks, 3 weeks, 4 weeks, and 5 weeks later. The original growth rate of VX-2 carci- noma in each group was calculated. Results ： VX-2 carcinoma in liver was found successfully in ten rabbits of both Group A and Group B; for Group C, VX-2 carcinoma in liver was found successfully in seven rabbits . Conclusion： The original growth rate of VX-2 carcinoma is high by the methods in all the groups, and experimental period is short. The methods are effective for the research of liver neoplasm.