全反式维甲酸对HL-60细胞核干细胞因子mRNA表达的影响

Effects of ATRA on mRNA expression of nucleostemin in HL-60 cells

目的:检测全反式维甲酸(ATRA)诱导后HL-60细胞核干细胞因子(NS)mRNA表达的变化,探讨NS mRNA表达与细胞分化之间的关系。方法:从NS基因的3个变异体中筛选出共同序列设计特异性引物,用终浓度为0.1μmol/L、1μmol/L、10μmol/L的ATRA诱导分化HL-60白血病细胞48h,以不加ATRA的HL-60细胞作为对照,应用逆转录-聚合酶链式反应(RT-PCR)检测HL-60细胞NS mRNA表达水平的变化。结果:ATRA诱导HL-60细胞分化后NS mRNA表达均有所降低,0.1μmol/L、1μmol/L、10μmol/L浓度下NS mRNA表达量分别下降3.72%±0.84%、58.70%±4.98%和61.60%±2.26%。其中1μmol/L和10μmol/L组与对照组和0.1μmol/L组比较,差异均有统计学意义(P均<0.05)。结论:NS可能与白血病细胞分化有关,参与调控细胞分化过程。

Aim： To determine the expression of nucleostemin （NS） mRNA in HL-60 cells after induced differentiation, and to approach the potential mechanism between NS mRNA and pathogenesis of acute leukemia. Methods：The common sequence was screened out from three variants of NS gene and the primers were designed. HL-60 cells were induced and differentiated by ATRA in 0. 1 μmol/L, 1 μmol/L,and 10 μmol/L concentration groups for 48 hours, and the NS mRNA expression was detected in HL-60 cells by RT-PCR. The same detection was performed in the undifferentiated HL-60 cells of control group. Results ： The expression of NS mRNA in different concentration of ATRA decreased than that of control group. After induced and differentiated by ATRA of 0.1μmol/L, 1 μmol/L, and 10 μmol/L concentration, the expression of NS mRNA in HL-60 cells decreased 3.72% ± 0. 84% , 58.70% ± 4.98% , and 61.60% ± 2.26% ,respectively. And NS mRNA expression decreased significantly in 1 μmol/L, 10 μmol/L groups compared with control group and 0.1 μmol/L group （ P 〈 0.05 ）. Conclusion ： NS may be in relation to the differentiation process of leukemia cells.