人膀胱移行细胞癌组织中八聚体转录因子4蛋白表达

Expression of octamer binding factor 4 in human transitional cell carcinoma tissue of bladder

目的:检测人膀胱移行细胞癌组织中核转录因子4(Oct4)蛋白的表达情况,并探讨其在膀胱移行细胞癌发生发展中的作用。方法:采用免疫组织化学SP法检测44例膀胱移行细胞癌(Ⅰ级7例,Ⅱ级27例,Ⅲ级10例)、2例非典型增生、19例癌旁组织和15例正常膀胱黏膜组织中Oct4蛋白的表达情况。结果:膀胱移行细胞癌和癌旁组织中Oct4蛋白的阳性表达率分别为63.6%(28/44)和36.8%(7/19),非典型增生和正常膀胱黏膜上皮组织中无Oct4表达,差异有统计学意义(P<0.05)。且膀胱移行细胞癌组织中Oct4蛋白的表达与分化程度相关(P<0.05)。结论:Oct4蛋白的表达可速膀胱移行细胞的恶性转化。

Aim ： To detect the expression of octamer biading factor 4 （Oct4） in human transitional cell carcinoma tissue, and investigate the relationship between Oct4 and the factors of clinical pathology. Methods：SP immunohistochemical method was used to detect the expression of Oct4 in 44 cases of transitional cell carcinoma（7 cases of grade Ⅰ ,27 cases of grade Ⅱ , 10 cases of gradeⅢ ） , 2 cases of atypical hyperplasia, 19 cases of pericarcinomatous tisue, and 15 cases of mucous membrane of urinary bladder. Results ： The Oct4 protein expression rate in transitional cell carcinoma tissue of bladder was 63.6% （28/44） , in periearcinomatouse tissue was 36.8% （7/19）. No expression of Oct4 in atypical hyperplasia and raucous membrane was observed. There was significant difference on Oct4 positive expresison rate among four tissues （P 〈 0.05）. The expression rate of Oct4 in bladder carcinoma tissue was negatively correlated with pathological grade （P 〈 0.05 ）. Conclusion ：The Oct4 protein may promote transitional cell carcinogenesis of bladder.