宫颈癌组织中凋亡相关蛋白激酶的表达

Expression of death-associated protein kinase in cervical cancer and cervical intraepithelial neoplasia tissue

目的:检测宫颈癌及子宫颈癌前病变组织中凋亡相关蛋白激酶(DAPK)蛋白的表达情况。方法:用免疫组织化学SP法检测52例宫颈癌、60例宫颈上皮内瘤变(CIN)及正常宫颈鳞状上皮组织中DAPK蛋白的表达。结果:正常宫颈、CIN和宫颈癌组织中DAPK蛋白阳性率分别为100%(20/20)、75.0%(45/60)和40.4%(21/52),差异有统计学意义(χ2=27.310,P<0.05)。CINⅠ组织中DAPK蛋白阳性率高于CINⅢ组织(P<0.05)。DAPK表达与宫颈癌临床分期有关(P<0.05),而与宫颈癌的病理类型、组织学分级和淋巴结转移等因素无关(P>0.05)。结论:DAPK作为一种抑癌基因,可能参与了宫颈癌的发生发展,有可能成为一个有价值的宫颈癌分子标志物。

Aim ： To detect the expression of death-associated protein kinase （DAPK） protein in cervical carcinoma and cervical intraepithelial neoplasia （CIN） tissue. Methods ：The expression of DAPK was detected by immunohistochemistry in 52 cases of cervical cancer and 60 cases of CIN tissue. Results：The positive rate of DAPK protein in normal cervical epithelia, CIN and cervical carcinoma tissue were 100% （20/20）,25.05% （45/60） and 40.4% （21/52）,respectively, there were significant differences（χ^2 = 27. 310,P 〈 0.05 ）. The positive rate of DAPK in CINⅢ tissue was higher than that in CIN Ⅰ tissue （P 〈0.05）. There was statistical association of the positive rate of DAPK protein with the stages of cervical cancer,but not with the pathological categories, histological grades and lymph node status（P 〉 0.05）. Gonclusion：DAPK protein may play an important role in cervical cancer development and progression. It may be a valuable cervical cancer marker.