恶性胸腔积液患者全身及病灶局部氧化抗氧化状态的研究

Study on oxidative/antioxidative status in patients with malignant pleural effusion

目的观察恶性胸腔积液(MPE)患者全身及病灶局部氧化抗氧化状态及生物大分子的氧化损伤。方法对28例MPE患者采用菲罗啉比色法检测胸水及血浆总抗氧化能力(TAC);采用硫代巴比妥酸比色法检测血浆丙二醛(MDA)的含量;采用单细胞凝胶电泳法检测胸水肿瘤伴随淋巴细胞(TALs)和外周血单个核细胞(PBMCs)的DNA损伤情况。结果MPE患者血浆TAC明显低于健康对照组(P<0.01),胸水TAC显著低于自身血浆TAC(P<0.01),血浆MDA明显高于健康对照组(P<0.01),血浆PBMCs的彗星率明显高于健康对照组(P<0.01),TALs的彗星率显著高于自身PBMCs彗星率(P<0.01)。MPE患者血浆TAC与MDA之间呈负相关(r=-0.516,P<0.01),胸水TAC与TALs彗星率之间呈负相关(r= -0.538,P<0.01),血浆TAC与PBMCs彗星率之间呈负相关(r=-0.426,P<0.05),血浆MDA与PBMCs彗星率之间呈正相关(r=0.467,P<0.05)。结论MPE患者全身及肿瘤局部存在氧化应激,以肿瘤局部氧化应激更加明显,氧化应激造成的TALs的DNA氧化损伤可能与TALs的功能障碍有关。

Objective To study the oxidative/antioxidative status and the level of oxidative damage of cellular macromolecules, such as DNA, in patients with malignant pleural effusion （MPE）. Methods The total antioxidant capacity （TAC） of plasma and pleural effusion supernatant, and malondialdehyde （MDA） of plasma were measured by colorimetry; and the DNA damage of tumor associated lymphocytes （TALs） and peripheral blood mononuclear cells （PBMCs） was detected by single cell gel electrophoresis assay in 28 untreated patients with MPE. Results TAC of plasma was remarkably lower in patients with MPE than in healthy persons （P 〈0.01）. TAC of MPE supernatant was significantly lower than that of plasma in these patients （P 〈0.01）. MDA of plasma was significantly higher in patients with MPE than in healthy persons （P 〈0.01）. The number of DNA damaged cell percentage （comet percentage） of PBMCs was higher in patients with MPE than in healthy persons （P 〈0.01）. Within these patients, comet percentage of TALs was significantly higher than that of PBMCs （P 〈0.01）. The negative correlations were observed between TAC of plasma and MDA of plasma （r = -0.516, P 〈0.01）, between TAC of MPE supernatant and comet percentage of TALs （r = -0.538, P 〈0.01） and between TAC of plasma and comet percentage of PBMCs （r = -0.426, P 〈0.05） in patients with MPE. A positive correlation was observed between MDA of plasma and comet percentage of PBMCs （r = 0.467, P 〈0.05） in patients with MPE. Conclusion Patients with MPE suffered from oxidative stress systemically and in cancer site, especially in cancer site. The DNA damage of TALs may be involved in its dysfunction.