额叶皮层神经干细胞定向诱导分化类神经元的超微结构观察

Study on the ultrastructure of directional differentiation neuron-like cells of temporal lobe cerebral cortex neuron stem cell （NSC）

目的研究大鼠额叶皮层神经干细胞（NSC）定向诱导分化类神经元细胞过程中的超微结构变化。方法取Wistar出生24h新生鼠额叶脑组织加入神经生长因子进行干细胞的原代及继代培养SABCNestin鉴定并定向培养，于1、3、7d进行扫描电镜观察。结果神经干细胞诱导分化第7天的类神经元细胞扫描电镜观察可见胞体饱满，有树枝状分支，末端可见鸭蹼状膨大的生长；透射电镜下观察可见细胞胞质中有大量的粗面内质网、线粒体与高尔基复合体，脂滴糖原颗粒及微丝、微管，核膜、核仁清楚。结论大脑额叶皮层神经干细胞经定向诱导分化形态学上能够分化成类神经元细胞结构。

Objective To evaluate the ultrastructure on frontal lobe cerebral cortex neuron stem cell （NSC） in the process of directional differentiation neuron-like cells. Method Newborn Wistar animal in 24 hour was used, and the frontal lobe cerebral cortex tissue was scraped, primary generation and secondary culture with nerve nutrition factor were conducted. Immunochemistry SABC method was used to identify Nestin. Scan electron microscope （ SEM ） sample was prepared and observed on cover glass which taken from the raise board contain directional differentiation neuron at 1,3,7 day. Results Nearly mature, full soma, dendritic branches, duck palm shape apical cone on the terminal were obviously observed on SEM at 7 days. Some synapse type structure appeared on the cell surface. Organelles, massive RER, Golgi apparatus and the fat drop glycogen pellet was rich on TEM at 7 days. Microfilament and microtubule were in line, big and round nucleolus were clear. All these neuron-like cells characteristic were obvious and easy to see. Conclusions This study indicates that the frontal lobe cerebral cortex nerve stem cell can be directional induced differentiate to neuron-like cells.