Rho激酶及血红素氧合酶在自发性高血压大鼠阴茎海绵体中的表达

Expression of Rho-kinase and Heme Oxygenase in the Corpus Cavernosum of Spontaneous Hypertensive Rats

目的：探讨NOS／NO、HO／CO、RhoA／Rho激酶等信号通路在自发性高血压大鼠（SHR）阴茎海绵体中的表达及相互关系。方法：健康成年雄性SPF级SHR与对照组WKY大鼠各7只，16周龄，体重250-300g。麻醉后颈动脉和海绵体内插管连续监测平均动脉压（MAP）和海绵体内压（ICP）。利用电刺激海绵体神经，记录ICP／MAP比值变化。利用免疫组化和Western印迹方法分析ROCK2、HO-2、eNOS在阴茎海绵体中的表达变化。结果：SHR组在利用电刺激海绵体神经后ICP／MAP比值升高不明显（P〉0．05），海绵体组织中ROCK2蛋白表达水平升高显著（P=0．017），HO-2表达水平则降低显著（P=0.006）。HO-2主要位于阴茎海绵体的平滑肌细胞及神经细胞内，eNOS则主要位于阴茎海绵体血管内皮细胞，两者在SHR组表达明显降低。结论：NOS／NO、HO／CO、RhoA／Rho激酶与SHRED有关，并且可能互相影响。

Objective： To investigate the expression of Rho-kinase and heme oxygenase and the interaction among NOS/NO, HO/CO and RhoA/Rho-kinase in the corpus cavemosum of spontaneous hypertensive rats （SHRs）. Methods： A series of electric stimuli were applied to the corpus cavemosum nerves of 7 SHRs, the changes of ICP/MAP observed continuously and the expressions of ROCK2, HO-2 and eNOS analyzed by Western blot and immunohistochemistry. Another 7 WKY rats were taken as controls. Resuits： Compared with the controls, ICP/MAP did not rise obviously （ P 〈 0.05 ）, HO-2 dropped sharply （ P = 0.006） and the expression of the ROCK2 protein was elevated markedly （ P = 0. 017 ） in the SHRs. Immunohistochemistry showed HO-2 to be distributed in the smooth muscle cells and nervous cells of the corpus cavemosum, and eNOS mainly in the vascular endotbelial cells. The expressions of HO-2 and eNOS were obviously reduced in the SHRs. Conclusion： NOS/NO, HO/CO and RhoA/Rho-kinase are related with erectile dysfunction in SHRs and may interact on one another.