钙调蛋白抑制剂对小鼠精子获能影响的研究

Calmodulin Antagonists Are Involved in Mouse Sperm Capacitaton

目的:探讨钙调蛋白是否参与小鼠精子获能过程。方法:用50、100、200μmol/L浓度的钙调蛋白抑制剂W7和10、20、30μmol/L浓度的钙调蛋白抑制剂卡米达佐(CZ)分别与小鼠精子孵育2h,通过金霉素染色法计算出B型精子百分率。并用100μmol/L钙调蛋白抑制剂W7和10μmol/LCZ分别与小鼠精子孵育2h后,再加入5μmol/L孕酮诱发顶体反应,计算精子顶体反应百分率。结果:不同浓度的W7和CZ作用小鼠精子,B型精子百分率呈浓度依赖性方式下降,与对照组比较,均有显著性差异(P<0.05)。顶体反应率与对照组相比均有极显著差异(P<0.01)。结论:钙调蛋白参与小鼠精子获能,是精子获能过程中的关键蛋白。

Objective： To investigate the possible involvement of calmodulin in mouse sperm capacitation. Methods. Calmodulin antagnnists W7 at the concentrations of 50, 100 and 200μmol/L and calmidazolium （CZ） at the concentrations of 10, 20 and 30μmol/ L, were coincubated with mouse sperm for 2 hours, respectively. The percentage of pattern B sperm was measured by chloroteracycline staining. Then the sperm were coincubated with 100μmol/L W7 or 10μmol/L calmidazolium （CZ） before acrosome reaction was induced by 5μmol/L progesterone and evaluated by the same method. Results： After the treatment of the sperm with different concen-trations of CZ or W7, the percentages of pattern B sperm decreased in a dose-dependent manner, significantly different from the control （P 〈 0.05 ）. There was a statistic difference in the rate of acrosome reaction between the experiment and the control group （P 〈 0.01 ）. Conclusion ： Calmodulin is a key protein involved in mouse sperm capacitation.