大肠癌中RECK基因表达及其临床病理意义

Expression and Clinicopathological Significance of RECK Gene in Colorectal Cancer

探讨RECK基因在大肠癌中的表达及其与大肠癌侵袭、转移及预后的关系。采用免疫组织化学SP法检测136例大肠癌组织及20例正常大肠黏膜组织中RECK基因的表达，并分析其与大肠癌临床病理因素的关系。结果显示，RECK基因在136例大肠癌组织中的阴性表达率为36．8％，在20例大肠正常黏膜中的阴性表达率为0（P〈0.01）；在I＋Ⅱ期与Ⅲ＋Ⅳ期的低表达率为65．9％和88．0％（P〈0．01）；在T1＋T2期与T3＋T4期两组的低表达率分别为68．3％和91．8％（P〈0．01）；在N0期与N1＋N2期两组的低表达率分别为61．4％和90．2％（P〈0．01）；在M0与M1两组的低表达率为77．8％和100％（P〈0．05）；RECK基因低表达组（5年生存率为37．3％）较高表达组（5年生存率为69．2％）预后差（P〈0.01）。结果表明，RECK基因在大肠癌中的低表达与肿瘤分期、肿瘤大小、淋巴结转移和器官转移有关，在大肠癌的侵袭、转移中发挥重要作用。

The objective of this study was to probe the expression of RECK gene and its clinical significance in colorectal cancer. The expressions of RECK in 136 cases of colorectal cancer were detected by immunohis-tochemical assay and their corelations with clinicopathological factors were analyzed. Results showed that among the 136 cases of colorectal cancer,the RECK negative expression rate was 36.8% ,and that was 0 of 20 normal tissues（ P〈0.01）. The low-expression rates were 65.9% and 88% in stage I ＋ Ⅱ and Ⅲ＋Ⅳ stages,respectively（ P〈0.01） ,and 68.3% and 91.8% in the group of T1 ＋T2 and T3＋T4 stages（ P〈0.01）, and 61.4% and 90.2% in the group of No and N1＋N2 stages（ P〈0.01） ,and 77.8% and 100% in the group of M0 and M1 （ P 〈0.05）. The RECK low-expression group（5-year overall survival rate.37.3%） had a worse prognosis than that of high-expression group（5-year overall survival rate： 69.2%）（ P〈0.01）. Above results show that RECK low-expression in colorectal cancer has positively related with the stage,the tumor size, lymph-node metastasis and distant metastasis. RECK low-expression may play a key role in the invasion and metastasis of colorectal cancer.