利用PCR技术专化性检测水稻细菌性条斑病菌

Specific detection of Xanthomonas oryzae pv.oryzicola by PCR techniques

设计水稻细菌性条斑病菌的专化性引物,并建立相应的PCR检测体系,分别对31株水稻细菌性条斑病菌和15株水稻白叶枯病菌及其它相关菌株进行了测试。结果表明,建立的PCR检测体系可专化性检测水稻细菌性条斑病菌,而水稻白叶枯病菌和其它菌株均没有扩增信号。检测灵敏度可以达到20个细菌菌体,从自然发病和人工接种发病的水稻种子成功地检测出条斑病菌。实现了对水稻细菌性条斑病菌的快速和专化性检测。

A pair of specific primers was designed and used for detection of Xanthomonas oryzae pv. oryzicola （Xooc）, the rice bacterial leaf streak pathogen. The results showed： all tested Xooc strains（31 isolates） were specific detected, 15 strains of Xanthomonas oryzae pv. oryzae and other tested bacteria strains had not any amplification signal. The detection limit was 20 cells. Xooc was successfully detected from the seeds harvest from the natural infected and artificially inoculated rice plants.