玉米弯孢叶斑病菌致病类型分化研究

Pathogenicity differentiation of Curvularia lunata

利用鉴别寄主和同工酶技术,对采自黑龙江、吉林、辽宁和河北4省玉米弯孢叶斑病菌菌株的致病类型进行分析。研究表明,鉴别寄主技术将26个菌株的致病类型划分为4种类型,其中强和中等致病类型主要分布在辽宁省瓦房店市、吉林省公主岭市和河北省保定市。玉米弯孢叶斑病菌在酯酶、苹果酸脱氢酶、过氧化物酶、多酚氧化酶、超氧化物歧化酶同工酶谱存在差异,不同菌株间的某些同工酶谱带数差异显著。

Pathogenic type of Curvularia lunata from Heilongjiang, Jilin, Liaoning and Hebei provinces was analyzed. Twenty-six isolates of C. lunata were divided into four groups by differential hosts. High and moderate pathogenic types were distributed in Wafangdiang city in Liaoning province, Gongzhuling city in Jilin province and Baodin city in Hebei province. A significant diversity of C. lunata was found according to the difference of isozymes including esterase, peroxidase, polyphenol oxidase, superoxide dimutase and malate dehydrogenase. The obvious difference in numbers of bands of isozymes were dectcted.