摘要
应用电镜观察、DAS-ELISA以及RT-PCR检测,从症状表现黄化、环斑的云南蝴蝶兰(Phalaenopsis amabilis)病样中分离得到的一个病毒分离物Tospo-Pha。该分离物粒子近球形、具包膜、直径约90nm,与番茄斑萎病毒属(Tospovirus)的西瓜银色斑驳病毒(Watermelon silver mosaic virus,WSMoV)/花生芽坏死病毒(Groundnut bud necrosis virus,GBNV)复合抗血清呈强阳性反应,分子检测发现该分离物SRNA5′末端序列与CaCV大岩桐分离物(CaCV-Gloxinia)同源性最高(91.0%),在系统进化树中与CaCV聚于同一分支。上述结果表明,从云南蝴蝶兰中分离到的Tospo-Pha属于Tospovirus病毒。
Using electron microscopy, DAS-ELISA and RT-PCR, a virus isolate （Tospo-Pha） was detected from Phalaenopsis amabilis showing yellow ringspot symptoms in Yunnan. Tospo-Pha had quasi-spherical, enveloped shape with diameters of 90 nm, reacted positively to compound antibody of Watermelon silver mosaic virus （WSMoV）/Groundnut bud necrosis virus （GBNV）. The S RNA 5＇-termini sequence of Tospo-Pha had highest identity （91.0 % ） with that of CaCV isolate Gloxinia （CaCV-Gloxinia）. The Tospo-Pha clustered with other CaCV isolates in phylogenetic tree. These results indicated Tospo-Pha belonged to the genus Tospovirus.
出处
《植物病理学报》
CAS
CSCD
北大核心
2008年第1期31-34,共4页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(30160005
30660008)
