摘要
用鸡空肠弯曲菌(Campytobacecriejun:)对1日龄SPF(Spciic-pathogenfrce)雏鸡进行感染实验。随机将60只1日龄SPF雏鸡分为4组,那和对照组,每组15只。分别用含菌7.5X108CFU/ml、7.5x104CFU/ml,7.5×102CFU/ml布氏肉汤菌液经口时t、J、I组接种。接种后分别于2、4、6,8、10日踏踏机取3只/组、经剖构观察病理变化;从心血、肝、胆汁、脾、肠内容物分离病原菌,测量肠道内物空肠弯曲菌菌数。得出以下结论;经口接种3.75x102CFU空场弯曲菌即可造成1日龄SPF雏鸡感染发病;感染鸡的主要病理变化为肝脏表面有点状,条索状或片状出血,同时伴有形状不规则大小不等的黄白色坏死灶,盲肠膨大充满气泡,十二指肠、空肠、直肠粘膜有散在的小出血点,首选检苗材料为胆汁、其次是脾、肝等器宫。
The experimental infection to 1-day-old specific-pathogen-free (SPF) chick. was conducted by an isolate of C. jejuni Sixty chick. were randomly divided into four roups ( I , I , I and contral). Group t I , 1 , were inocultaed orally. .with the doses. of 7. 5 X 10, colony-forming uits (CFU)ml , 7. 5 X 104 CFU/ml and 7. 5 X 102 CfU/ml respectively. Three chicks in each group were killed at the interval of 2 , 4, 6,8, 10 day. after, infection the pathological lesions were observed and isolation assay of the organ- ism was made of heart blood ,livers . spleens ,bile and intestines. Viable counts of C. jejuni in contents of the digestive tract were done. The ineffective experiment showed that 1-day-old SPF chick. could be in- fected with C. jejuni of 3. 75X 102 CFU and showed the obvious pathological lesinos. The local necrosis and hemorrhage of liver were the major pathological characteristics. In addition , ceca distended with froth and the foci of hemorrhages. of mucosa of duodenum jejunum , ileum. and Colon were ofter . found. The results also suggested the first potion. of material for isolation. is bile , then spleens and livers.
出处
《黑龙江畜牧兽医》
CAS
北大核心
1997年第11期4-8,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
黑龙江省科学技术计划项目