基于ANEP染料荧光光谱迁移的单波长心脏光学标测系统

An Optical Mapping System Based on Spectral Shift of Voltage-Sensitive Dyes

近几年来,光学标测技术已经成为心脏电生理研究中一种非常重要的手段。它利用对嵌在细胞膜上的电压敏感染料随着膜电位变化而产生的荧光光谱迁移进行成像,来进行心律失常与电击除颤等电生理研究。文章测量了常用的电压敏感染料di-4-ANEPPS的荧光光谱,并根据该染料的光谱迁移,设计了一套包括一个通用CCD相机的单波长光学标测系统,可以达到较高的时空分辨率。记录心肌细胞中的电兴奋传导过程,从而可以为今后国内心律失常作用机制的研究工作提供一个有力的工具。

Recently,non-invasive optical methods to monitor transmembrane electrical potential using voltage sensitive dyes have been applied widely in the studies of normal and pathological heart rhythms and defibrillation.In the present paper,the authors measured the excitation and the emission spectra of the voltage-sensitive dyes di-4-ANEPPS bound to phospholipid bilayer membranes.And according to the spectral shift of di-4-ANEPPS,the authors presented an optical mapping system combining a DALSA CCD camera and a LED light source.Using this optical mapping system,the authors could record the action potential duration of the heart cells with high spatial and temporal resolutions.It can be a powerful tool in the study of cardiac arrhythmia mechanisms.