蒙药牛胆粉与羊胆粉中胆酸类成分的分析测定

The Analysis of the Content of Bile Acids in Ox-Bile Powder and Goat Gall Powder of Mongolian Medicine

胆酸与硫酸加热产生脱水反应,形成共轭双键,在紫外区产生特征吸收峰。用紫外-分光光度法测定了牛胆粉与羊胆粉中胆酸的含量。测定线性范围为0·0033～0·0167mg·mL-1(r=0·9997),加样回收率及RSD分别为98·48%,1·79%,96·46%,2·50%。5批次样品测定表明:牛胆粉中胆酸的含量在40·85%～43·03%之间,羊胆粉在30·88%～32·64%之间。牛胆粉精密度RSD为2·41%,羊胆粉精密度RSD为2·92%。稳定性试验在8h之内测定牛胆粉RSD为0·55%,羊胆粉RSD为0·59%。重复性试验牛胆粉RSD为2·11%,羊胆粉RSD为2·68%。方法简便、快速、便于推广应用,且可有效地控制牛胆粉与羊胆粉的内在质量。

The present study was to detect and compare the content of bile acids in ox bile powder and goat gall powder of Mongolia medicine by UV.Cholic acid with sulphuric acid were heated and dehydrated,and they produced conjugated double bond.The conjugated bond showed the same absorption peak in the ultraviolet range.The method of ultraviolet spectrophotometry can be used to detect and compare the content of bile acids in ox bile powder and goat gall powder.The result showed that the linear range was 0.003 3-0.016 7 mg·mL^-1（r=0.999 7）.The average recovery（n=5） of standard addition method of ox bile powder and goat gall powder was 98.48%（RSD=1.79%） and 96.46%（RSD=2.50%） respectively.The result of determination of five different samples showed that the content of bile acids in ox bile powder and goat gall powder was 40.85%-43.03% and 30.88%-32.64% respectively.The RSD of the analysis of ox bile powder and goat ball powder was 2.40% and 2.92% respectively,the RSD of stationary test of ox bile powder and goat ball powder in eight hours was 0.55% and 0.59% respectively,and the RSD of reproducibility of the analysis of ox bile powder and goat ball powder was 2.11% and 2.68% respectively.The method was simple,accurate,fast and easy to generalize and apply in many fields.It can be used to control the quality of ox bile powder and goat gall powder.