寻常型银屑病皮损中Th17细胞相关因子的表达

Expression of the cytokines associated with Th17 cell in the psoriatic lesions

目的:研究Th17细胞相关因子在银屑病发病机制中的作用。方法:用逆转录-聚合酶链反应法(RT-PCR)半定量分析寻常型银屑病患者皮损区、非皮损区及正常人皮肤组织中Th17细胞相关因子IL-17和IL-23(p19/p40)、IL-6mRNA的表达。结果:银屑病患者皮损中IL-17、IL-23p19、IL-23p40和IL-6的mRNA平均相对表达量分别为1.231±0.843、1.166±0.142、1.125±0.104和1.186±0.222。皮损组织中该4种细胞因子mRNA表达水平均高于非皮损组和正常皮肤组,非皮损组四者表达高于正常对照组,差异均有统计学意义(均P<0.05)。结论:Th17细胞相关因子在银屑病患者皮损中过度表达,提示Th17型细胞因子在银屑病的免疫发病机制中可能起着非常重要的作用。

Objective： To investigate the pathogenic effects of Th17 eytokines in psoriasis. Methods： Using RT - PCR technique to semiquantitatively analyze the mRNA expression of IL- 17, IL - 23 （p19/p40） and IL - 6 in lesional and non - lesional skin of psoriatic patients and normal skin tissue. Results： The mRNA expression of IL- 17, IL - 23 （p19/p40） and IL - 6 in psoriatic lesional skin was upregulated compared with that of non - lesional skin and normal skin. Meanwhile, the mRNA expression of IL- 17, IL-23 （p19/p40） and IL-6 in psoriatic non - lesional skin was higher than that of normal skin （ P both 〈 0.05）. Conclusion： The over - expression of cytokines associated with Th17 cell in the lesion from psoriatic patients may play an important role in the immunopathogenesis of psoriasis.