首段尿和尿道拭子标本应用PCR法检测生殖支原体的比较

Comparison of first void urine and swab specimens for the detection of mycoplasma genitalium by polymerase chain reaction

目的:比较性病门诊男性患者PCR尿道拭子和首段尿标本对生殖支原体(Mg)的检测。方法:采集非淋菌性尿道炎(NGU)患者及无尿道炎就诊者的泌尿生殖道拭子标本及首段尿液标本,分别运用2对引物的PCR法检测Mg。结果:NGU组和无尿道炎者的拭子标本、首段尿标本Mg检出率分别为8.68%、5.90%;3.92%、3.92%。NGU组两种标本Mg的检出率间有统计学差异,P<0.05;无尿道炎就诊者的两种标本的Mg检出率间无统计学差异(P>0.05)。结论:首段尿标本检测生殖支原体可作为泌尿生殖道拭子标本检测的补充,提高临床检出率,还可作为无症状者的筛查手段。

Objective： To compare urogenital swab and first void urine （FVU） specimens from male patients for the detection of Mycoplasma genitalium （Mg） infections by PCR. Methods： Swab specimens and first void urine （FVU） were collected from male patients with nongonococcal urethritis （NGU） and STD clinic attendees without urethritis, and were detected for Mg by two kinds of PCR. Results： The detection rates of Mg using urogenital swab specimens in NGU patients and subjects without urethritis were 8.68% and 5.90% respectively, and the detection rates were 3.92% and 3.92% respectively in the two groups when FVU was tested. In NGU patients, the detection rate was significantly higher in swab specimens compared to FVU. But no difference was found in the two different specimens for subjects without urethritis. Conclusion： FUV can be used as a supplement of swab specimen for the detection of Mg in NGU patients and as a screening tool for non - NGU subjects.