p16和PCNA在宫颈上皮内瘤变及宫颈癌中的表达

Expression of p16 and PCNA protein in cervical intraepithelial neoplasia and cervical carcinoma

目的探讨p16和PCNA表达产物在宫颈上皮瘤样病变及宫颈癌中表达的意义。方法对正常宫颈鳞状上皮、宫颈上皮内瘤变(CIN)和宫颈癌组织共95例,采用免疫组织化学S-P法,对宫颈癌变过程中p16和PCNA蛋白进行研究,将结果进行统计分析。结果p16蛋白在CIN中的表达高于正常宫颈上皮(P<0.01),在宫颈癌中的表达也高于正常宫颈上皮(P<0.01);PCNA蛋白在CIN中的表达高于正常宫颈上皮(P<0.01),在宫颈癌中的表达也高于正常宫颈上皮(P<0.01)。p16和PCNA两种蛋白在CIN和宫颈癌中的表达无明显差异。结论p16和PCNA蛋白的表达与宫颈癌的发生有关,提示这两种蛋白有可能成为高危人群早期筛查的一种免疫组化指标。

Objective To investigate the significance of expression of p16 and PCNA in cervical intraepithelial neoplasia （CIN） and cervical carcinoma. Methods The expression of p16 and PCNA proteins of 95 cases of normal cervical tissue, cervical intraepithelial neoplasia and cervical carcinoma was detected by SP immunohistochemical staining and the results were correlate with the results. Results The expression of p16 was significantly higher in CIN than in normal cervixes （P〈0.01）, It was significantly higher in cervical carcinomas than in normal cervixes（P〈0.01 ） ;The expression of PCNA was significantly higher in CIN than in normal cervixes（P〈0.01 ）.It was significantly higher in cervical carcinomas than in normal cervixes （P〈0.01）.No statistical difference of expression in cervical carcinomas and CIN was found between p16 and PCNA protein. Conclusion The results indicated that the expression of p16 and PCNA protein was associated with the carcinogenesis of cervical cancer. It suggested that p16 and PCNA expression may be taken as effective immunohistochemical markers for early detection.