摘要
目的:研究以反相高效液相色谱(RP-HPLC)法同时测定烧伤涂膜中黄芩苷、大黄素含量的方法。方法:采用Lichrospher C18(150 mm×4.6 mm)柱,以甲醇-0.2%膦酸为流动相梯度洗脱,检测波长280 nm,流速为1.0ml/min。结果:黄芩苷在0.712~3.00 mg/ml范围内线性关系良好,r=0.9982,平均回收率为97.74%,相对标准差(RSD)为2.37%(n=9)。大黄素在0.212~0.895 mg/ml范围内线性关系良好,r=0.999 8,平均回收率为97.67%,RSD为1.92%(n=9)。结论:此法操作简便,结果可靠,重现性好,可作为该产品的质量控制方法。
Objective: To determine baicalin and emodin in burn membrane by reverse phase highpressure liquid chromatography (RP-HPLC). Methods : A Lichrospher C 18 ( 150 mm× 4.6 mm) column was used, with methanol 0.2% phosphoric acid as the mobile phase for gradient elution. The detection wavelength was 280 nm and the flow rate was 1.0 mL/min. Results : Baicalin had a good linearity within the range of 0. 712 - 3.00 mg/ ml (r = 0, 998 2) , and the average recovery was 97.74% with RSD of 2.37% (n = 9). Emodin had a good linearity within the range of 0. 212 - 0,895 mg/ml ( r = 0.999 8) ,and the average recovery was 97.67% with RSD of 1.92% ( n = 9). Conclusion: RP-HPLC, simple, accurate and reproducible, can be used for controlling the quality of burn membrane.
出处
《医学研究生学报》
CAS
2008年第3期235-236,241,共3页
Journal of Medical Postgraduates
基金
江苏省六大人才高峰基金资助项目(批准号:06-B-028)
关键词
反相高效液相色谱
烧伤涂膜
梯度洗脱
黄芩苷
大黄素
Reverse phase high-pressure ,liquid chromatography
Burn membrane
Gradient elution
Baicalin
Emodin