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异氟醚麻醉对大鼠海马蛋白质组的影响 被引量：1

Effects of isoflurane on hippocampal proteome on the hippocnmpus proteome after in rats
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摘要 目的研究异氟醚麻醉后大鼠海马蛋白质组的变化。方法将10只8月龄SD大鼠随机均分为异氟醚组(I组)和对照组(C组)。I组大鼠以1·2%异氟醚吸入麻醉2h,C组吸氧对照。于麻醉处理或吸氧对照结束后24h取海马组织行双向凝胶电泳和质谱分析以鉴定差异蛋白点。结果双向凝胶电泳结果显示I和C组差异蛋白质点共有17(4/13)个,MALDI-TOF-MS分析后鉴定出12种蛋白质。结论异氟醚麻醉可以引起大鼠海马蛋白质组变化,这些蛋白质变化可能具有一定的神经元保护作用。 Objective To investigate the alteration of hippocampus proteome after isoflurane anesthesia in rats. Methods Ten 8-month-old SD rats were randomly divided into control group（group C）and isofluroame group（group I）. The rats in group C only inhaled mixed gas containing 40% oxygen for two hours. The rats in group I were inhaled 1.2% isoflurane in 40% oxygen for two hours. The hippocampus of the rat was cut down for two dimensional gel electrophoresis （2-DE） 24 h after anesthesia. The different protein spots were analyzed by means of mass chromatographic analysis. Results There were 17 （4/13）different protein spots between group I and group C, of which 12 protein spots of all spots were identified by MALDI-TOF-MS. Conclusion The study shows that isoflurane may cause the alteration of hippocampal proteome in rats,and this change may be related to the neuron protection of isoflurane preconditioning.
作者 段开明 欧阳文 夏月峰 张文静 李冠
机构地区 中南大学湘雅三医院麻醉科
出处 《临床麻醉学杂志》 CAS CSCD 2008年第3期239-241,共3页 Journal of Clinical Anesthesiology
基金 国家自然科学基金(编号30771052) 湖南省自然科学基金(编号07555039)
关键词 异氟醚 大鼠 海马 蛋白质组 Isoflurane Rat Hippocampus Proteome
分类号 R614 [医药卫生—麻醉学]
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参考文献7

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共引文献78

同被引文献11

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临床麻醉学杂志
2008年 第3期

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