摘要
本试验通过筛选抗原性较好的多杀性巴氏杆菌,经禽胚培养增殖细菌,灭活后加入免疫增强剂左旋咪唑和亚硒酸钠维生素E,制成油乳剂强化苗。试验表明,每头份含抗原量100亿,左旋咪唑和亚硒酸钠维生素E合用组免疫效果最佳。注射本苗后,鸡群第4天产生免疫应答,第7天即可产生坚强的免疫力,试管凝集抗体效价达到1:86,攻毒保护率达83.3％,第14天即可达100％,免疫期6个月。免疫期内攻毒保护率为97.6％,当试管凝集抗体效价为1:40时,80％的试验鸡能抵御C_(48-1)的攻击。临床上免疫接种4个鸡场12批商品蛋鸡共14.5万羽,有效地控制了该病的流行。
The antigenic Pasteuralla multocida was selected and cultured by poultry embryas. 'I he oil - eniul-sion enhanced vaccine was prepared by mixing inactivated antigen with two immunologic enhancers and oil - adjuvants in a certain proportion. The vaccine contained 10 bacteria per dose and two immunologic enhancers had op-timun immune effect. The layers raised immune response 4 days post inoculation(p. i) and showed good protection 7 days p. i. When the antibody liter determined by tube agglutination test was 1:86 the vaccinated layers protec lion rate against C48 -1 strain was 83. 3% . The layers obtained 100% protection 14 days p. i and the immune protection period of the vaccine lasted 6 months. The average protection rate in the immune period was 97. 6% . The vaccinated layers had 80% protection rate when the antibody liter was 1:40. The application of the vaccine to 12 conlmous batches about 145 thousand layers in four farms showecl it had no side 'effect but good efficacy.
基金
上海市教委资助项目
关键词
禽霍乱
强化苗
保护率
免疫期
Fowl cholera
Enhanced inactivated vaccine
Protection rate
Immune protection period
