转化生长因子-β1基因多态性在严重急性呼吸综合征发生及愈后肺间质纤维化中的作用

Roles of transforming growth factor-Ill gene polymorphisms in severe acute respiratory syndrome and the interstitial lung fibrosis after recovered

目的 研究TGF-β1基因多态性在SARS-冠状病毒（SARS-CoV）感染及愈后肺间质纤维化中的作用。方法 选取65例SARS康复人员、37例医护人员和66例健康人员，进行TGF-β1基因多态性与SARS-CoV易感性及与SARS愈后肺间质纤维化关系的分析。采用病例一病例研究设计，在SARS康复患者中分析TGF-β1基因多态性与SARS愈后肺间质纤维化的关系。采用聚合酶链反应一直接测序法（PCR-SBT）进行TGF-β1基因多态性检测，数据行t检验和卡方检验。结果 TGF-β1基因＋869和＋915位点的基因多态性在SARS康复人群、医护人员和对照人群分布差异无统计学意义，未见该基因两位点的多态性连锁与SARS-CoV易感性有关。SARS愈后人群的肺间质纤维化改变与TGF-β1基因＋869和＋915位点的基因多态性无关，也未见该基因＋869和＋915位点的多态性连锁与肺间质纤维化相关。结论 TGF-β1基因＋869和＋915位点的基因多态性可能与SARS—CoV易感性无关；SARS康复人员的肺间质纤维化可能与TGF-β1＋869和＋915基因多态性无关。

Objective To investigate the roles of transforming growth factor（TGF）-β1 gene polymorphisms in severe acute respiratory syndrome-coronavirus （SARS-CoV） infection and the interstitial lung fibrosis after recovered. Methods Sixty-five recovered SARS patients, 37 health care workers and 66 healthy controls were enrolled in this case-case study. The association between genetic polymorphisms of TGF-β1 and susceptibility to SARS or interstitial lung changes after SARS recovered was carried out. Polymerase chain reaction-sequencing based typing（PCR-SBT） method was used to determine the polymorphisms of TGF-β1 gene at locus ＋869 and ＋915. Data were analyzed using t test and chi square test. Results There was no significant association of TGF-β1 gene polymorphisms at locus ＋ 869 and ＋ 915 in recovered SARS patients, health care workers and healthy controls. And gene linkage of this two loci was not related with SARS-CoV susceptibility. Furthermore, no association between interstitial lung changes in recovered SARS patients and TGF-β1 gene polymorphisms or genetic linkage of this two loci. Conclusions It may not be related between TGF- β1 gene polymorphisms at locus ＋869 and ＋915 and SARS-CoV susceptibility. And interstitial lung changes in recovered SARS patients may not be influenced by TGF-β1 gene polymorphisms.