禽副粘病毒2型(APMV-2)血凝素单克隆抗体的制备与初步应用

Preparation and Identification of the Monoclonal Antibody Specific to Hemagglutinin of Avian Paramyxovirus Type 2

A hybridoma cell line 1G4A7 secreting monoclonal antibody (McAb) specific to hemagglutinin of avian paramyxovirus type 2 (APMV-2) was developed by fusing the spleen cells of APMV-2 immunized BAlb/c mice with SP2/0 myeloma cells. The immunoglobulin subclass of 1G4A7 was IgG1 with light chain κ and the affinity constant against APMV-2 was 1.02×1010. Identified by HI and indirect ELISA, the McAb titers in ascities were 10 log 2 and 1∶106 respectively. The McAb did not cross react with the common avian viruses, showing good specificity. There existed obvious differences in antigenitic relationship among APMV-2 viruses analyzed by HI and indirect ELISA using McAb 1G4A7.

A hybridoma cell line 1G4A7 secreting monoclonal antibody （McAb） specific to hemagglutinin of avian paramyxovirus type 2 （APMV-2） was developed by fusing the spleen cells of APMV-2 immunized BAlb/c mice with SP2/0 myeloma cells. The immunoglobulin subclass of 1G4A7 was IgG1 with light chain and the affinity constant against APMV-2 was 1.02 × 10^10. Identified by HI and indirect ELISA, the McAb titers in ascities were 10 log 2 and 1 ：10^6 respectively. The McAb did not cross react with the common avian viruses, showing good specificity. There existed obvious differences in antigenitic relationship among APMV-2 viruses analyzed by HI and indirect ELISA using McAb 1G4AT.