一种新型的应用于生物医学领域的数字热显微镜

Digital Thermal Microscope for Biomedical Application

本文基于非制冷焦平面探测器设计了一种新型的可应用于生物医学分析的数字热显微镜,该热显微镜可以获得长波红外显微热图像。研究了热显微镜系统噪声等效温差(NETD)和噪声等效辐射率差(NEED)模型。基于噪声等效温差模型,分析了提高系统温度分辨率的可行性方法。提出了一种基于单幅画面的非制冷焦平面探测器的非均匀校正算法,同时采用了直方图均衡化方法来提高图像的质量。基于VisualC++和已经完成的算法完成了整个系统的软件设计。实际物体的图像采集处理结果表明了该数字热显微镜设计的有效性和合理性。利用该热显微镜,可以完成微小物体的热分析,因此它将成为生物医学分析的有力手段,可以加速该领域的发展并具有广泛的应用前景。

In order to analyze the biomedical, we proposed a novel digital thermal microscope based on the uncooled focal plane detector, aiming to achieve the long-wave infrared microscope image, especially for biomedical analysis. Both the mathematical mode of noise equivalent temperature difference （ NETD ） and tile noise equivalent eradiation difference （NEED） were established for micro thermal imaging system. Based on the mathematical model, some measures were taken to increase the system temperature resolution. Furthermore the uncooled focal plane arrays has inherent non-uniformities, so we proposed an adaptive algorithm that can complete NUC by only one frame. Results of our thermal microscope have proved that NUC can weaken striping noise greatly and plateau histogram equalization can further enhance the image quality. The software for the thermal microscope is provided based on Visual C＋＋ and the methods mentioned above. Results of real thermal image experiments have shown that the digital thermal microscope is designed successfully and achieves good perfm＇manee. With the thermal microscope, minute sized thermal analysis can be achieved. Thus it will become an effective means for biomedical analysis, and it can also accelerate the development of methods for biomedical engineering.