干旱响应转录因子DREB1A基因导入水稻光温敏核不育系的研究

Studies on Transformation of Photo-sensitive and Thermo-sensitive Genic Male Sterile Rice with Dehydration Responsive Transcription Factor Gene DREB1A

以成熟胚诱导的愈伤组织作为农杆菌转化的受体材料,将诱导型启动子rd29A驱动的拟南芥DREB1A基因导入粳型光温敏核不育系水稻4008S,共获得67株再生苗.再生苗经0.75mg/L除草剂草铵膦涂布筛选,有62株再生苗表现出对草铵膦抗性.PCR检测抗性苗中DREB1A基因,结果全为阳性.挑选部分进行Southern检测,结果表明目的基因已经整合到水稻基因组中.在干旱胁迫下,转基因水稻当代(T1代)植株的电导率显著低于非转基因对照植株(P<0.05),脯氨酸含量显著高于对照植株(P<0.05),证明DREB1A基因能提高水稻对干旱胁迫的耐受性.

Sixty-seven regenerated plantlets were obtained by agrobacterium-mediated transformation of A rabidopsis thaliana DREB1A gene driven by stress-inducible promoter rd29A into photo-sensitive and thermo-sensitive genic male sterile （P/TGMS） line 4008S. They were spread with 0.75 mg/L glufosinate on leaves,sixty-two of them were approved to be herbicide resistant. PCR analysis showed that all of the resistant plantlets possessed the DREB1A gene and the Southern blot confirmed that the target gene had been integrated into the rice genome. Under the drought stress,the relative conductivity of the transgenic plants were lower than that of the control plants（P〈0.05）,and the content of proline in the transgenic plants were higher than that in the control plants（P〈0.05）. They implied that the expression of DREB1A gene can improve the drought tolerance of rice.