中国人GFPT2基因单核苷酸多态性与北方汉族人群2型糖尿病发生的相关性

Relationship between the single nucleotide polymorphism of GFAT2 and type 2 diabetic mellitus in Han people of Shandong

目的研究新诊断2型糖尿病(T2DM)易感基因谷氨酰胺∶果糖-6-磷酸酶酰胺转移酶(GFPT)单核苷酸多态性与中国北方汉族人群T2DM的关系。方法实验分T2DM组和正常对照组,用酚/氯仿法提取其外周血DNA行PCR扩增,分离纯化目的条带后直接测序。结果GFPT2基因的第14外显子中SNP7位点等位基因为C/T,携带等位基因T的个体T2DM发病率较携带等位基因CC的个体显著升高,两组该等位基因T频率有统计学差异(P<0.01)。结论GFPT2等位基因可能是中国北方汉族人群T2DM主要易感基因位点之一。

Objective To investigate the relationship between the single nucleotide polymorphism of GFPT2 and type 2 diabetic mellitus （T2DM） in Han people of Shandong. Methods Peripheral blood DNA in subjects from T2DM group and control group was extracted and polymerase chain reaction（ PCR） was pedormed. Results The aUele gene of SNP7 in the 14th extron of GFPT2 gene was C/T, the allele T frequency in T2DM group was significantly higher than that in control group （P 〈 0.01 ）. Conclusions The allele gene T of GFPT2 may be the major susceptibility gene of T2DM of Han people in Shandong.