摘要
目的8-异前列腺素F2a（8-iso—PGF2a）作为反映缺氧缺血-再灌注后脂质过氧化作用的指标具有准确、特异、敏感的特性。而新型抗氧化剂N-乙酰半胱氨酸（NAC）具有清除氧自由基抗氧化的功能。本研究旨在探讨实验性缺氧缺血性脑损伤大鼠尿与脑组织8-iso—PGF2a相关性及尿8-iso—PGF2a能否作为监测早期脂质过氧化作用的生化指标及NAC的治疗效果。方法7日龄SD新生大鼠60只随机分为对照组、缺氧缺血组和NAC组。NAC组给予NAC0．1mg／（g·d），于缺氧缺血前3d灌胃，共3d；缺氧缺血组给予等体积生理盐水，对照组不做相应处理。应用ELISA法测定各组大鼠尿与脑组织8-iso—PGF2a含量。结果缺氧缺血组尿和脑组织含量分别为（101．5±22．8）ng／mmol．cr和（237．2±11．3）ng／g，明显高于对照组（26．5±11．6）ng／mmol．cr和（90.8±18．7）ng／g（P〈0．01），NAC组（40．1±9．9）ng／mmol．cr和（110．5±12．4）ng／g，明显低于缺血组（P〈0．01），尿与脑组织8-iso—PGF2a水平相关（r=0．823，P〈0．01）。结论急性缺氧缺血性脑损伤时尿8-iso—PGF2a含量产生增加，NAC能有效的降低尿8-iso—PGF2a水平，可用于治疗缺氧缺血性脑损伤。
Objective 8 -iso- prostaglandin F2a （8 -iso- PGF2a） is an index that can sensitively,truthly and specificany reflect peroxidation caused by reperfusion. N- acetlycysteine（NAC） acts as an effective scavenger for free radical cleaning in the body. This study aimed to analyze the correlation of 8 - iso - PGF2a level between urine and brain tissue, and to investigate the rapeutic effect of NAC, and the possibility of level of 8 - iso - PGF2a in urine as biochemicalindex. Methods Seven - day - old Spraygue - Dawley（SD） rats were randomly divided into 3 groups ： hypoxia - ischemia, NAC and control group. Rats in the NAC group were given dastric lavage with NAC 0. 1 mg/（ g · d） for three days, Rats in hypoxia- ischemia group were given an equal volune of normal saline, and control group had no administra -tion. The 8 -iso -PGF2a contents in urine and brain tissue were determined by ELISA. Results Compared with the control group, 8 - iso - PGF2a contents in urine and braintissue of the hypoxia - ischemia group were significantly higher [ （26. 5 ± 11. 6 ） ng/mmol, cr vs （ 101.5 ± 22.8 ） ng/mmol, cr and （ 90. 8 ± 18.7） ng/g vs（237. 2 ± 11.3） ng/g] ,both P 〈0. 01. In the NAC group, 8 - iso - PGF2a concentration of urine and brain tissue were （40.1 ± 9. 9 ） ng/mmol, cr and （ 110. 5 ± 12.4 ）ng/g respectively, lower than those in the hypoxia - ischemia group（ P 〈 0. 01 ）. The 8 - iso - PGF2a levels of urine and brain tissue were positively correlated （ r = 0. 823, P 〈 0. 01 ）. Conclusions Thelevel of 8 - iso - PGF2a increased in both urine and brain tissue of rats with acute brain hypoxia - ischemia, NAC reduces the level of 8 - iso - PGF2a in urine. Therefore it may be used in treatment of brain hypoxia - ischemia.
